UNIGE document Scientific Article
previous document  unige:30076  next document
add to browser collection
Title

The critical cis-acting element required for IMD2 feedback regulation by GDP is a TATA box located 202 nucleotides upstream of the transcription start site

Authors
Escobar-Henriques, Mafalda
Daignan-Fornier, Bertrand
Published in Molecular and Cellular Biology. 2003, vol. 23, no. 17, p. 6267-78
Abstract Guanylic nucleotides are essential cellular players, and the critical enzyme in their tightly regulated synthesis in Saccharomyces cerevisiae is encoded by the IMD2 gene. The transcription of IMD2 is subject to general repression by nutrient limitation through the cis nutrient-sensing element. It is also subject to specific feedback regulation by the end products of the guanylic nucleotide synthesis pathway. The critical cis element for this latter mechanism is the guanine response element (GRE), a TATAATA sequence which is located 202 nucleotides upstream of the transcription initiation site and which functions as the IMD2 TATA box. We show that the GRE functions in conjunction with a 52-nucleotide stretch near the transcription start site. This very unusual promoter structure ensures low, basal expression of IMD2 and the recruitment of TFIID to the GRE in response to guanylic nucleotide limitation.
Keywords Base SequenceDrug Resistance, Fungal/geneticsFeedback, Physiological/geneticsGene Expression Regulation, FungalGuanine/metabolismGuanosine Diphosphate/metabolismIMP Dehydrogenase/genetics/metabolismMolecular Sequence DataMycophenolic Acid/pharmacologyNucleotides/geneticsPromoter Regions, GeneticRegulatory Sequences, Nucleic AcidResponse Elements/geneticsSaccharomyces cerevisiae/drug effects/genetics/metabolismSaccharomyces cerevisiae Proteins/genetics/metabolismTATA BoxTranscription Factor TFIID/genetics/metabolismTranscription Initiation Site
Identifiers
PMID: 12917347
Full text
Article (Published version) (555 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe Collart Martine (microbiologie et médecine moléculaire) (81)
Project FNS: 3100- 059199.99
Citation
(ISO format)
ESCOBAR-HENRIQUES, Mafalda, DAIGNAN-FORNIER, Bertrand, COLLART, Martine. The critical cis-acting element required for IMD2 feedback regulation by GDP is a TATA box located 202 nucleotides upstream of the transcription start site. In: Molecular and Cellular Biology, 2003, vol. 23, n° 17, p. 6267-78. https://archive-ouverte.unige.ch/unige:30076

186 hits

0 download

Update

Deposited on : 2013-10-01

Export document
Format :
Citation style :