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Saccharomyces cerevisiae Ccr4-not complex contributes to the control of Msn2p-dependent transcription by the Ras/cAMP pathway

Labarre, J
Published in Molecular microbiology. 2002, vol. 43, no. 4, p. 1023-37
Abstract The Ccr4-Not complex is a global regulator of transcription that affects genes positively and negatively and is thought to modulate the activity of TFIID. In the present work, we provide evidence that the Ccr4-Not complex may contribute to transcriptional regulation by the Ras/cAMP pathway. Several observations support this model. First, Msn2/4p-dependent transcription, which is known to be under negative control of cAMP-dependent protein kinase (PKA), is derepressed in all ccr4-not mutants. This phenotype is paralleled by specific post-translational modification defects of Msn2p in ccr4-not mutants relative to wild-type cells. Secondly, mutations in various NOT genes result in a synthetic temperature-sensitive growth defect when combined with mutations that compromise cells for PKA activity and at least partially suppress the effects of both a dominant-active RAS2Val-19 allele and loss of Rim15p. Thirdly, Not3p and Not5p, which are modified and subsequently degraded by stress signals that also lead to increased Msn2/4p-dependent activity, show a specific two-hybrid interaction with Tpk2p. Together, our results suggest that the Ccr4-Not complex may function as an effector of the Ras/cAMP pathway that contributes to repress basal, stress- and starvation-induced transcription by Msn2/4p.
Keywords Culture MediaCyclic AMP/metabolismCyclic AMP-Dependent Protein KinasesDNA-Binding Proteins/genetics/metabolismFungal Proteins/genetics/metabolismGene Expression Regulation, FungalGlucose/pharmacologyHSP70 Heat-Shock Proteins/geneticsMutagenesisProtein Processing, Post-TranslationalRibonucleases/genetics/metabolismSaccharomyces cerevisiae/drug effects/genetics/metabolismSaccharomyces cerevisiae Proteins/genetics/metabolismSignal TransductionTranscription Factors/genetics/metabolismRas Proteins/metabolism
PMID: 11929548
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Research group Caractérisation génétique et biochimique du complexe Ccr4-Not conservé (81)
Swiss National Science Foundation: 3100-059199
Swiss National Science Foundation: 3100-49808.96
(ISO format)
LENSSEN, Eve et al. Saccharomyces cerevisiae Ccr4-not complex contributes to the control of Msn2p-dependent transcription by the Ras/cAMP pathway. In: Molecular microbiology, 2002, vol. 43, n° 4, p. 1023-37. doi: 10.1046/j.1365-2958.2002.02799.x https://archive-ouverte.unige.ch/unige:30070

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Deposited on : 2013-10-01

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