Scientific article

Saccharomyces cerevisiae Ccr4-not complex contributes to the control of Msn2p-dependent transcription by the Ras/cAMP pathway

Published inMolecular microbiology, vol. 43, no. 4, p. 1023-1037
Publication date2002

The Ccr4-Not complex is a global regulator of transcription that affects genes positively and negatively and is thought to modulate the activity of TFIID. In the present work, we provide evidence that the Ccr4-Not complex may contribute to transcriptional regulation by the Ras/cAMP pathway. Several observations support this model. First, Msn2/4p-dependent transcription, which is known to be under negative control of cAMP-dependent protein kinase (PKA), is derepressed in all ccr4-not mutants. This phenotype is paralleled by specific post-translational modification defects of Msn2p in ccr4-not mutants relative to wild-type cells. Secondly, mutations in various NOT genes result in a synthetic temperature-sensitive growth defect when combined with mutations that compromise cells for PKA activity and at least partially suppress the effects of both a dominant-active RAS2Val-19 allele and loss of Rim15p. Thirdly, Not3p and Not5p, which are modified and subsequently degraded by stress signals that also lead to increased Msn2/4p-dependent activity, show a specific two-hybrid interaction with Tpk2p. Together, our results suggest that the Ccr4-Not complex may function as an effector of the Ras/cAMP pathway that contributes to repress basal, stress- and starvation-induced transcription by Msn2/4p.

  • Culture Media
  • Cyclic AMP/metabolism
  • Cyclic AMP-Dependent Protein Kinases
  • DNA-Binding Proteins/genetics/metabolism
  • Fungal Proteins/genetics/metabolism
  • Gene Expression Regulation, Fungal
  • Glucose/pharmacology
  • HSP70 Heat-Shock Proteins/genetics
  • Mutagenesis
  • Protein Processing, Post-Translational
  • Ribonucleases/genetics/metabolism
  • Saccharomyces cerevisiae/drug effects/genetics/metabolism
  • Saccharomyces cerevisiae Proteins/genetics/metabolism
  • Signal Transduction
  • Transcription Factors/genetics/metabolism
  • Ras Proteins/metabolism
  • Swiss National Science Foundation - Genetic and biochemical analysis of transcriptional regulation inyeast
  • Swiss National Science Foundation - 3100-49808.96
Citation (ISO format)
LENSSEN, Eve et al. Saccharomyces cerevisiae Ccr4-not complex contributes to the control of Msn2p-dependent transcription by the Ras/cAMP pathway. In: Molecular microbiology, 2002, vol. 43, n° 4, p. 1023–1037. doi: 10.1046/j.1365-2958.2002.02799.x
Main files (1)
Article (Published version)
ISSN of the journal0950-382X

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