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Scientific article
English

Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex

Published inEMBO journal, vol. 21, no. 3, p. 355-364
Publication date2002
Abstract

The RING finger protein CNOT4 is a component of the CCR4-NOT complex. This complex is implicated in repression of RNA polymerase II transcription. Here we demonstrate that CNOT4 functions as a ubiquitin-protein ligase (E3). We show that the unique C4C4 RING domain of CNOT4 interacts with a subset of ubiquitin-conjugating enzymes (E2s). Using NMR spectroscopy, we detail the interaction of CNOT4 with UbcH5B and characterize RING residues that are critical for this interaction. CNOT4 acts as a potent E3 ligase in vitro. Mutations that destabilize the E2-E3 interface abolish this activity. Based on these results, we present a model of how E3 ligase function within the CCR4-NOT complex relates to transcriptional regulation.

Keywords
  • Amino Acid Sequence
  • DNA-Binding Proteins/genetics
  • Fungal Proteins/genetics
  • Ligases/genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Repressor Proteins/genetics
  • Ribonucleases
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors/genetics
  • Transcription, Genetic
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligases
Citation (ISO format)
ALBERT, Thomas K et al. Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. In: EMBO journal, 2002, vol. 21, n° 3, p. 355–364. doi: 10.1093/emboj/21.3.355
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ISSN of the journal0261-4189
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