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The NOT, SPT3, and MOT1 genes functionally interact to regulate transcription at core promoters

Published in Molecular and Cellular Biology. 1996, vol. 16, no. 12, p. 6668-76
Abstract Previous studies demonstrated that mutations in the Saccharomyces cerevisiae NOT genes increase transcription from TATA-less promoters. In this report, I show that in contrast, mutations in the yeast MOT1 gene decrease transcription from TATA-less promoters. I also demonstrate specific genetic interactions between the Not complex, Mot1p, and another global regulator of transcription in S. cerevisiae, Spt3p. Five distinct genetic interactions have been established. First, a null allele of SPT3, or a mutation in SPT15 that disrupts the interaction between Spt3p and TATA-binding protein (TBP), allele specifically suppressed the not1-2 mutation. Second, in contrast to not mutations, mutations in MOT1 decreased HIS3 and HIS4 TATA-less transcription. Third, not mutations suppressed toxicity due to overexpression of TBP in mot1-1 mutants. Finally, overexpression of SPT3 caused a weak Not- mutant phenotype in mot1-1 mutants. Collectively, these results suggest a novel type of transcriptional regulation whereby the distribution of limiting TBP (TFIID) on weak and strong TBP-binding core promoters is regulated: Mot1p releases stably bound TBP to allow its redistribution to low-affinity sites, and the Not proteins negatively regulate the activity of factors such as Spt3p that favor distribution of TBP to these low-affinity sites.
Keywords Adenosine TriphosphatasesDNA Helicases/geneticsDNA-Binding ProteinsFungal Proteins/geneticsGene Expression Regulation, FungalImmediate-Early Proteins/geneticsMutationNuclear Receptor Subfamily 4, Group A, Member 2Promoter Regions, Genetic/geneticsSaccharomyces cerevisiae/geneticsSaccharomyces cerevisiae ProteinsTATA-Binding Protein Associated FactorsTranscription Factors/genetics
PMID: 8943321
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Research group Groupe Collart Martine (microbiologie et médecine moléculaire) (81)
Project FNS: 31-39690.93
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COLLART, Martine. The NOT, SPT3, and MOT1 genes functionally interact to regulate transcription at core promoters. In: Molecular and Cellular Biology, 1996, vol. 16, n° 12, p. 6668-76. doi: 10.1128/mcb.16.12.6668 https://archive-ouverte.unige.ch/unige:30057

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Deposited on : 2013-10-01

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