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Scientific article
English

The NOT, SPT3, and MOT1 genes functionally interact to regulate transcription at core promoters

ContributorsCollart, Martine
Published inMolecular and cellular biology, vol. 16, no. 12, p. 6668-6676
Publication date1996
Abstract

Previous studies demonstrated that mutations in the Saccharomyces cerevisiae NOT genes increase transcription from TATA-less promoters. In this report, I show that in contrast, mutations in the yeast MOT1 gene decrease transcription from TATA-less promoters. I also demonstrate specific genetic interactions between the Not complex, Mot1p, and another global regulator of transcription in S. cerevisiae, Spt3p. Five distinct genetic interactions have been established. First, a null allele of SPT3, or a mutation in SPT15 that disrupts the interaction between Spt3p and TATA-binding protein (TBP), allele specifically suppressed the not1-2 mutation. Second, in contrast to not mutations, mutations in MOT1 decreased HIS3 and HIS4 TATA-less transcription. Third, not mutations suppressed toxicity due to overexpression of TBP in mot1-1 mutants. Finally, overexpression of SPT3 caused a weak Not- mutant phenotype in mot1-1 mutants. Collectively, these results suggest a novel type of transcriptional regulation whereby the distribution of limiting TBP (TFIID) on weak and strong TBP-binding core promoters is regulated: Mot1p releases stably bound TBP to allow its redistribution to low-affinity sites, and the Not proteins negatively regulate the activity of factors such as Spt3p that favor distribution of TBP to these low-affinity sites.

Keywords
  • Adenosine Triphosphatases
  • DNA Helicases/genetics
  • DNA-Binding Proteins
  • Fungal Proteins/genetics
  • Gene Expression Regulation, Fungal
  • Immediate-Early Proteins/genetics
  • Mutation
  • Nuclear Receptor Subfamily 4, Group A, Member 2
  • Promoter Regions, Genetic/genetics
  • Saccharomyces cerevisiae/genetics
  • Saccharomyces cerevisiae Proteins
  • TATA-Binding Protein Associated Factors
  • Transcription Factors/genetics
Funding
  • Swiss National Science Foundation - 31-39690.93
Citation (ISO format)
COLLART, Martine. The NOT, SPT3, and MOT1 genes functionally interact to regulate transcription at core promoters. In: Molecular and cellular biology, 1996, vol. 16, n° 12, p. 6668–6676. doi: 10.1128/mcb.16.12.6668
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Article (Published version)
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Identifiers
ISSN of the journal0270-7306
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