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Title

NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization
Authors
Collart, Martine
Struhl, Kevin
Published in Genes & development. 1994, vol. 8, no. 5, p. 525-37
Abstract The yeast HIS3 TR and TC TATA elements support basal transcription, but only TR can respond to transcriptional activators. Four genes, NOT1(CDC39), NOT2(CDC36), NOT3, NOT4, act as general negative regulators and preferentially affect TC-dependent transcription. Allele-specific suppression, a two-hybrid interaction, and biochemical confractionation suggest that NOT1 and NOT2 are nuclear proteins associated in a discrete, 500-kD complex. NOT4 interacts with NOT1 and NOT3 in the two-hybrid assay, and overexpression of NOT3 or NOT4 suppresses not1 and not2 mutations. Repression by the NOT proteins is not attributable to inhibition of transcriptional activators, does not involve the CYC8/TUP1 negative regulatory complex, and is distinct from repression by nucleosomes or by the SPT4, 5, 6 proteins that affect chromatin structure. We propose that the NOT protein inhibit the basic RNA polymerase II transcription machinery, possibly by affecting TFIID function.
Keywords Base Sequence — Cell Cycle Proteins — Fungal Proteins/genetics — Gene Expression Regulation, Fungal — Genes, Fungal — Hydro-Lyases/genetics — Molecular Sequence Data — Mutation — Promoter Regions, Genetic — Repressor Proteins/genetics — Saccharomyces cerevisiae/genetics — Saccharomyces cerevisiae Proteins — TATA Box — Transcription Factors/genetics — Transcription, Genetic
Identifiers
PMID: 7926748
Full text
Article (Published version) (2.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research group Caractérisation génétique et biochimique du complexe Ccr4-Not conservé (81)
Citation
(ISO format) 		COLLART, Martine, STRUHL, Kevin. NOT1(CDC39), NOT2(CDC36), NOT3, and NOT4 encode a global-negative regulator of transcription that differentially affects TATA-element utilization. In: Genes & development, 1994, vol. 8, n° 5, p. 525-37. doi: 10.1101/gad.8.5.525 https://archive-ouverte.unige.ch/unige:30054

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Deposited on : 2013-10-01

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