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Scientific article
English

Functional Activity of a Biotinylated Human Neurokinin 1 Receptor Fusion Expressed in the Semliki Forest Virus System

Contributors

Lundstrom, Kenneth; Vargas, Alfredo; Allet, Bernard

Published in

Biochemical and biophysical research communications, vol. 208, no. 1, p. 260-266

Publication date

1995

Abstract

The 1.3 S biotinylatable subunit of Proprionibacterium shermanii transcarboxylase complex was fused to the C-terminus of the human neurokinin 1 receptor gene and introduced into the Semliki Forest virus expression vector pSFV1. RNA transcribed from pSFV1-NK1-biot and pSFV-Helper2 was coelectroporated into BHK cells permitting in vivo packaging of recombinant virus. Infection of BHK and CHO cells with SFV-NK1-biot virus yielded high level of the fusion receptor as detected by metabolic labeling, immunoblotting with streptavidin alkaline phosphatase and binding to substance P. Like native receptor, the biotinylated receptor fusion was able to stimulate Ca2+ mobilization in infected CHO cells,indicating functional coupling to guanine-nucleotide-binding proteins.

Keywords

  • Hamster ovary cells
  • Escherichia-coli
  • Substance-P
  • Peptides
  • Binding
  • Protein
  • Vectors

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

LUNDSTROM, Kenneth, VARGAS, Alfredo, ALLET, Bernard. Functional Activity of a Biotinylated Human Neurokinin 1 Receptor Fusion Expressed in the Semliki Forest Virus System. In: Biochemical and biophysical research communications, 1995, vol. 208, n° 1, p. 260–266. doi: 10.1006/bbrc.1995.1332

Identifiers

Additional URL for this publication

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WBK-45NJVRR-PG-1&_cdi=6713&_user=779890&_orig=search&_coverDate=03%2F08%2F1995&_sk=997919998&view=c&wchp=dGLzVzz-zSkWA&md5=9f1fda76ba4977a0aeb29fbbb4c36809&ie=/sdarticle.pdf

Journal ISSN

0006-291X
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Technical informations

Creation

21/09/2009 16:11:10

First validation

21/09/2009 16:11:10

Update

14/03/2023 15:12:41

Status update

14/03/2023 15:12:41

Last indexation

29/10/2024 12:07:08
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