en
Scientific article
English

T-cell-receptor repertoire in chronic plaque-stage psoriasis is restricted and lacks enrichment of superantigen-associated V beta regions

Published inJournal of investigative dermatology, vol. 104, no. 5, p. 725-728
Publication date1995
Abstract

Preferential usage of certain T-cell receptors by the lymphocytic infiltrate in psoriasis might indicate the involvement of an antigen in the pathogenesis of this disease. However, to date there are no data on the complete T-cell-receptor V alpha and V beta repertoire in psoriatic patients. We therefore compared the usage of T-cell-receptor variable regions in blood and skin of 10 patients with chronic plaque-stage psoriasis by means of semiquantitative polymerase chain reaction. Additionally, HLA class II alleles were analyzed by means of sequence-specific oligonucleotide typing. A considerable restriction of the T-cell-receptor repertoire was observed in the skin, where up to 20% of the variable regions present in the blood were not detectable. This was true for both alpha- and beta-chains. However, no interindividually constant pattern of T-cell-receptor restriction was deducible. Inconsistently, a certain preferential usage of some beta chains occurred within the cutaneous compartment. This report on the complete T-cell-receptor V alpha and V beta repertoire in psoriasis documents the restricted receptor repertoire of infiltrating T cells and a lack of enrichment of superantigen-associated V beta regions. Thus superantigens seem not to play a pathogenetically relevant role in chronic plaque-stage psoriasis.

Keywords
  • Genetic Heterogeneity
  • HLA-DQ Antigens/genetics
  • HLA-DQ alpha-Chains
  • HLA-DR Antigens/genetics
  • HLA-DRB1 Chains
  • Humans
  • Immunoglobulin Variable Region/blood/*immunology
  • Polymerase Chain Reaction/methods
  • Psoriasis/blood/*metabolism
  • Receptors, Antigen, T-Cell, alpha-beta/*immunology
  • Skin/chemistry
  • Superantigens/*physiology
Affiliation Not a UNIGE publication
Citation (ISO format)
BOEHNCKE, Wolf-Henning et al. T-cell-receptor repertoire in chronic plaque-stage psoriasis is restricted and lacks enrichment of superantigen-associated V beta regions. In: Journal of investigative dermatology, 1995, vol. 104, n° 5, p. 725–728. doi: 10.1111/1523-1747.ep12606966
Main files (1)
Article
accessLevelRestricted
Identifiers
ISSN of the journal0022-202X
470views
0downloads

Technical informations

Creation09/20/2013 4:49:00 PM
First validation09/20/2013 4:49:00 PM
Update time03/14/2023 8:26:17 PM
Status update03/14/2023 8:26:17 PM
Last indexation02/12/2024 8:34:14 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack