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Scientific article
English

The alpha-defensins HNP-1 and HNP-2 are dominant self-peptides presented by HLA class-II molecules in lesional psoriatic skin

Published inEJD. European journal of dermatology, vol. 14, no. 3, p. 142-145
Publication date2004
Abstract

Peptides bound to HLA class II molecules are involved in epidermal T-cell activation, an integral part in the pathogenesis of psoriasis. To characterize the peptide repertoire displayed, HLA class II molecules were isolated from human HaCaT keratinocytes and from a split skin specimen of a patient with plaque-type psoriasis. Human neutrophil peptides (HNP) 1 and 2, belonging to the alpha-subfamily of defensins, were identified as dominant HLA class II bound self-peptides by means of HPLC and subsequent electrospray ionization mass spectrometry in both cases. Assuming the characteristic intramolecular disulfide bonds remain intact, an HLA class II binding motif can be modelled for HNP-1 and -2. Both peptides inhibited superantigen-mediated T-cell activation in vitro. In conclusion, HNP-1 and -2 are dominant HLA class II bound self-peptides displayed by keratinocytes in psoriasis. They are capable of interfering with superantigen-mediated T-cell activation. This might be a hint towards a possible role of alpha-defensins in the down-regulation of inflammation.

Keywords
  • Adult
  • Cell Line/drug effects
  • Dose-Response Relationship, Drug
  • Female
  • HLA-DR Antigens/*immunology
  • Humans
  • Psoriasis/*immunology/pathology
  • Alpha-Defensins/*immunology/pharmacology
Affiliation Not a UNIGE publication
Citation (ISO format)
BOEHNCKE, Wolf-Henning. The alpha-defensins HNP-1 and HNP-2 are dominant self-peptides presented by HLA class-II molecules in lesional psoriatic skin. In: EJD. European journal of dermatology, 2004, vol. 14, n° 3, p. 142–145.
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ISSN of the journal1167-1122
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