en
Scientific article
English

T-cell receptor beta variable region (V beta) usage in cutaneous T-cell lymphomas (CTCL) in comparison to normal and eczematous skin

Published inJournal of cutaneous pathology, vol. 23, no. 4, p. 298-305
Publication date1996
Abstract

Cutaneous T-cell lymphomas (CTCL) are a heterogeneous group of lymphoproliferative disorders. We investigated the variable region (V beta) of the T-cell receptor (TCR) repertoire in CTCL and compared it to the V beta repertoire in normal and eczematous skin. We used a panel of 21 anti-V beta antibodies and investigated 84 biopsies of 71 CTCL patients (4 parapsoriasis en grandes plaques (PA), 1 lymphomatoid papulosis, 29 mycosis fungoides (MF), 13 Sezary syndrome (SS), 1 CD8+ CTCL, 11 pleomorphic CTCL (PLEO), 12 CTCL nor classified). Six biopsies of normal skin and 6 of eczematous skin lesions served as controls. We determined the frequency of the V beta in normal and inflamed skin and compared it to the percentage of the respective V beta in the malignant clone of the CTCL patients. The percentage of the V beta positive CD4+ cells in relation to the total number of T cells in normal skin and inflamed skin differed from the distribution of the V beta families in the peripheral blood mononuclear cells (PBMC). Out of 71 CTCL cases, the clone was identified in 23 (32%). We identified the following clones: 1 V beta 3.1 (16MF), 7 V beta 5.1 (1 CD8+ CTCL, 1 CTCL not classified, 1 MF, 1 PA, 3 SS), 1 V beta 6.7 (1 SS), 7 V beta 8.1/8.2 (2 CTCL not classified, 1 PLEO, 2 MF, 2 SS), 1 V beta 12.1 (1 PLEO), 3 V beta 17.1 (2 CTCL not classified, 1 MF), 2 V beta 22.1 (1 CTCL not classified, 1 MF), 1 TCR delta (SS). The frequency of the malignant clone V beta usage corresponded well to the repertoire of V beta in eczematous skin but not to the repertoire in PBMC. In 6 patients, the malignant clone was mainly localized in the epidermis. In 17 cases, the clone-specific cells were distributed in epidermis and dermis equally. A retrospective analysis showed that preferential epidermal homing of the clone was associated with a non-aggressive clinical course. The V beta usage of CTCL and eczema suggests a special cutaneous microenvironment which might be co-created by certain (bacterial?) superantigens. A preferential epidermal homing of the clone might have prognostic implications.

Keywords
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Eczema/*immunology/*pathology
  • Humans
  • Immunohistochemistry
  • Lymphoma, T-Cell/immunology/*pathology
  • Middle Aged
  • Receptors, Antigen, T-Cell, alpha-beta/*analysis/*immunology
  • Skin/*immunology/pathology
  • Skin Neoplasms/immunology/*pathology
  • T-Lymphocyte Subsets/*immunology
Affiliation Not a UNIGE publication
Citation (ISO format)
POTOCZNA, N. et al. T-cell receptor beta variable region (V beta) usage in cutaneous T-cell lymphomas (CTCL) in comparison to normal and eczematous skin. In: Journal of cutaneous pathology, 1996, vol. 23, n° 4, p. 298–305.
Identifiers
ISSN of the journal0303-6987
508views
0downloads

Technical informations

Creation09/20/2013 4:32:54 PM
First validation09/20/2013 4:32:54 PM
Update time03/14/2023 8:24:45 PM
Status update03/14/2023 8:24:45 PM
Last indexation01/16/2024 2:09:08 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack