en
Scientific article
English

Clonal disease in early cutaneous T-cell lymphoma

Published inDermatologic clinics, vol. 12, no. 2, p. 351-360
Publication date1994
Abstract

Our experience with 185 cases of T-cell dominated skin infiltrates demonstrates that the PCR-based method for detection of TCR gamma chain gene rearrangement in combination with temperature gradient electrophoresis can routinely be used for the demonstration of clonal T cells in formalin-fixed and paraffin-embedded biopsies of lesional skin. In contrast to Southern blot analysis, the amplification by PCR is nonradioactive, is not time consuming (approximately 3 days), can be performed using frozen or paraffin-embedded tissue, and allows additional molecular biologic analyses, such as sequencing. Furthermore, it offers the possibility to design patient-specific primers for monitoring of the disease activity. It also has to be concluded from our study that all available clinical, histologic, cytologic, immunophenotypical, and rearrangement studies have to be considered in order to establish the correct diagnosis.

Keywords
  • Blotting, Southern
  • Clone Cells
  • Diagnosis, Differential
  • Electrophoresis
  • Gene Rearrangement, T-Lymphocyte
  • Humans
  • Immunohistochemistry
  • Lymphoma, Non-Hodgkin/pathology
  • Lymphoma, T-Cell, Cutaneous/*diagnosis
  • Paraffin Embedding
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell/genetics
  • Receptors, Antigen, T-Cell, gamma-delta/genetics
  • Skin Neoplasms/*diagnosis
  • T-Lymphocytes
Affiliation Not a UNIGE publication
Citation (ISO format)
MIELKE, V. et al. Clonal disease in early cutaneous T-cell lymphoma. In: Dermatologic clinics, 1994, vol. 12, n° 2, p. 351–360.
Identifiers
ISSN of the journal0733-8635
441views
0downloads

Technical informations

Creation09/20/2013 4:32:53 PM
First validation09/20/2013 4:32:53 PM
Update time03/14/2023 8:24:44 PM
Status update03/14/2023 8:24:44 PM
Last indexation01/16/2024 2:09:05 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack