• Home
  1. Home
  2. C-type particles are inducible in Se-Ax, a continuous T-cell line from a patient with Sezary's syndrome
Scientific article
English

C-type particles are inducible in Se-Ax, a continuous T-cell line from a patient with Sezary's syndrome

Contributors

Kaltoft, K.; Bisballe, S.; Rasmussen, H. F.; Thestrup-Pedersen, K.; Boehncke, Wolf-Henning; Volker, H.; Sterry, Wolfram

Published in

Archives of dermatological research, vol. 280, no. 5, p. 264-267

Publication date

1988

Abstract

Se-Ax is a continuous mature T-cell line that we have established from a patient with Sezary's syndrome. An important finding was that the Se-Ax cell line required human serum for initial growth. Here we show that transfer of the permanent cell line to a medium deficient of human serum induces production of C-type retroviral-like particles with a unique morphology. Ultrastructurally, these particles are 120 nm in diameter with hexagonal shape, and show a small, centrally located round core of 30 nm. They are observed only extra-cellularly; typical budding, however, is not found. Both by serological testing and molecular analysis we substantiate the morphological conclusion that these particles do not represent common human or animal retroviruses. The inducibility of the particles may be a hint as to a possible endogenous origin.

Keywords

  • Antigens, Differentiation/analysis
  • Cell Line
  • DNA, Viral/analysis
  • Human T-lymphotropic virus 1/genetics
  • Humans
  • RNA-Directed DNA Polymerase/metabolism
  • Retroviridae/*isolation & purification/ultrastructure
  • Sezary Syndrome/immunology/*microbiology
  • T-Lymphocytes/classification/immunology/microbiology

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

KALTOFT, K. et al. C-type particles are inducible in Se-Ax, a continuous T-cell line from a patient with Sezary’s syndrome. In: Archives of dermatological research, 1988, vol. 280, n° 5, p. 264–267. doi: 10.1007/bf00440598

Identifiers

Additional URL for this publication

http://link.springer.com/article/10.1007%2FBF00440598

Journal ISSN

0340-3696
549views
0downloads

Technical informations

Creation

20/09/2013 16:32:53

First validation

20/09/2013 16:32:53

Update

14/03/2023 20:24:43

Status update

14/03/2023 20:24:43

Last indexation

30/10/2024 10:15:37
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated