Discrimination between immunoaccessory and phagocytic monocytes/macrophages of the skin in paraffin-embedded tissue by the monoclonal antibody Ki-M1P
|Published in||British journal of dermatology. 1993, vol. 129, no. 2, p. 124-130|
|Abstract||The variety of functions performed by monocytes and macrophages is reflected in their phenotypic diversity. Investigation of this complex system is facilitated by Ki-M1P, a new monoclonal antibody which recognizes a differentiation antigen on monocytes/macrophages in paraffin-embedded tissues. To test its usefulness as a pan-macrophage marker in the skin, we immunohistochemically analysed paraffin-embedded biopsy material from seven healthy individuals and 190 patients with a variety of dermatoses. Immunoreactivity was compared with results obtained with the antibodies KP-1, MAC-387, UCHL-1 and S-100. In normal skin, epidermal Langerhans cells were Ki-M1P-. Strong expression of this marker was detected on spindle-shaped as well as dendritic perivascular and intervascular macrophages. Pathological reaction forms such as giant cells and epithelioid cells in granulomatous dermatoses were also Ki-M1P+. The high specificity of Ki-M1P is reflected in the lack of reactivity with tumour cells in non-monocytic neoplasms and Langerhans cell histiocytosis. Thus, Ki-M1P is a useful marker for skin macrophages, discriminating between the immunoaccessory and the phagocytic compartments.|
|Keywords||Antibodies, Monoclonal — Antigens, Differentiation/*analysis — Dendritic Cells/immunology/physiology — Dermatitis/immunology/pathology — Epidermis/cytology/immunology — Granuloma/immunology/pathology — Histiocytosis, Langerhans-Cell/immunology/pathology — Humans — Langerhans Cells/immunology/physiology — Macrophages/*immunology/*physiology — Monocytes/*immunology/*physiology — Paraffin Embedding — *Phagocytosis — Phenotype — Skin/*cytology/*immunology — Skin Diseases/immunology/pathology — Skin Neoplasms/immunology/pathology — Xanthogranuloma, Juvenile/immunology/pathology|
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|BOEHNCKE, Wolf-Henning et al. Discrimination between immunoaccessory and phagocytic monocytes/macrophages of the skin in paraffin-embedded tissue by the monoclonal antibody Ki-M1P. In: British journal of dermatology, 1993, vol. 129, n° 2, p. 124-130. doi: 10.1111/j.1365-2133.1993.tb03513.x https://archive-ouverte.unige.ch/unige:29684|