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Title

Discrimination between immunoaccessory and phagocytic monocytes/macrophages of the skin in paraffin-embedded tissue by the monoclonal antibody Ki-M1P

Authors
Dorhage, K. W.
Harms, D.
Radzun, H. J.
Hauschild, A.
Sterry, Wolfram
Published in British Journal of Dermatology. 1993, vol. 129, no. 2, p. 124-130
Abstract The variety of functions performed by monocytes and macrophages is reflected in their phenotypic diversity. Investigation of this complex system is facilitated by Ki-M1P, a new monoclonal antibody which recognizes a differentiation antigen on monocytes/macrophages in paraffin-embedded tissues. To test its usefulness as a pan-macrophage marker in the skin, we immunohistochemically analysed paraffin-embedded biopsy material from seven healthy individuals and 190 patients with a variety of dermatoses. Immunoreactivity was compared with results obtained with the antibodies KP-1, MAC-387, UCHL-1 and S-100. In normal skin, epidermal Langerhans cells were Ki-M1P-. Strong expression of this marker was detected on spindle-shaped as well as dendritic perivascular and intervascular macrophages. Pathological reaction forms such as giant cells and epithelioid cells in granulomatous dermatoses were also Ki-M1P+. The high specificity of Ki-M1P is reflected in the lack of reactivity with tumour cells in non-monocytic neoplasms and Langerhans cell histiocytosis. Thus, Ki-M1P is a useful marker for skin macrophages, discriminating between the immunoaccessory and the phagocytic compartments.
Keywords Antibodies, MonoclonalAntigens, Differentiation/*analysisDendritic Cells/immunology/physiologyDermatitis/immunology/pathologyEpidermis/cytology/immunologyGranuloma/immunology/pathologyHistiocytosis, Langerhans-Cell/immunology/pathologyHumansLangerhans Cells/immunology/physiologyMacrophages/*immunology/*physiologyMonocytes/*immunology/*physiologyParaffin Embedding*PhagocytosisPhenotypeSkin/*cytology/*immunologySkin Diseases/immunology/pathologySkin Neoplasms/immunology/pathologyXanthogranuloma, Juvenile/immunology/pathology
Identifiers
PMID: 7654569
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BOEHNCKE, Wolf-Henning et al. Discrimination between immunoaccessory and phagocytic monocytes/macrophages of the skin in paraffin-embedded tissue by the monoclonal antibody Ki-M1P. In: British Journal of Dermatology, 1993, vol. 129, n° 2, p. 124-130. https://archive-ouverte.unige.ch/unige:29684

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Deposited on : 2013-09-20

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