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Title

Low molecular weight dextran sulfate binds to human myoblasts and improves their survival after transplantation in mice
Authors
Laumonier, Thomas
Pradier, Amandine
Hoffmeyer, Pierre
Kindler, Vincent Lucien
Menetrey, Jacques
Published in Cell Transplantation. 2013, vol. 22, no. 7, p. 1213-26
Abstract Myoblast transplantation represents a promising therapeutic strategy in the treatment of several genetic muscular disorders including Duchenne muscular dystrophy. Nevertheless, such an approach is impaired by the rapid death, limited migration, and rejection of transplanted myoblasts by the host. Low molecular weight dextran sulfate (DXS), a sulfated polysaccharide, has been reported to act as a cytoprotectant for various cell types. Therefore, we investigated whether DXS could act as a "myoblastprotectant" either in vitro or in vivo after transplantation in immunodeficient mice. In vitro, DXS bound human myoblasts in a dose-dependent manner and significantly inhibited staurosporine-mediated apoptosis and necrosis. DXS pretreatment also protected human myoblasts from natural killer cell-mediated cytotoxicity. When human myoblasts engineered to express the renilla luciferase transgene were transplanted in immunodeficient mice, bioluminescence imaging analysis revealed that the proportion of surviving myoblasts 1 and 3 days after transplantation was two times higher when cells were preincubated with DXS compared to control (77.9 ± 10.1% vs. 39.4 ± 4.9%, p = 0.0009 and 38.1 ± 8.5% vs. 15.1 ± 3.4%, p = 0.01, respectively). Taken together, we provide evidence that DXS acts as a myoblast protectant in vitro and is able in vivo to prevent the early death of transplanted myoblasts.
Identifiers
PMID: 23031415
Full text
Article (Published version) (765 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Faculté de médecine / Section de médecine clinique / Département de chirurgie
Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales
Research groups Thérapie cellulaire et troubles musculo-squelettiques (960)
Immunité innée non leucocytaire (928)
Citation
(ISO format) 		LAUMONIER, Thomas et al. Low molecular weight dextran sulfate binds to human myoblasts and improves their survival after transplantation in mice. In: Cell Transplantation, 2013, vol. 22, n° 7, p. 1213-26. https://archive-ouverte.unige.ch/unige:29648

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Deposited on : 2013-09-18

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