Regulation of the Abd-B gene in the bithorax complex of the fruit fly

ContributorsEl Bali, Yohan
Master program titleMaster en biologie orientation Génétique Développement et Evolution
Defense date2013

In order to monitor in Drosophila the expression of the iab - 8ncARN in vivo, I decided to introduce a sequence encoding the fluorescent protein " mCherry " in exon 3 of the iab - 8ncARN . The choice of this exon is dictated by the existence of an integration platform in the iab-6 domain that encodes exon 3 . I managed the integration of the reporter gene using the technique of recombineering and transformation of Drosophila with integrase phiC31 . I discovered that a truncated form of the iab - 8ncARN is expressed specifically in the secondary cells of glands of the reproductive system in males that become fluorescent . I have not yet succeeded to detect expression of mCherry in embryos.

Citation (ISO format)
EL BALI, Yohan. Regulation of the Abd-B gene in the bithorax complex of the fruit fly. 2013.
Main files (1)
Master thesis
  • PID : unige:29607

Technical informations

Creation09/10/2013 3:02:00 PM
First validation09/10/2013 3:02:00 PM
Update time03/14/2023 8:24:05 PM
Status update03/14/2023 8:24:05 PM
Last indexation01/29/2024 7:56:00 PM
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