en
Master
English

Characterization of Mutants Involved in Release of Heterochromatic Silencing after a Long Heat Stress

ContributorsAntico, Djan
Master program titleMaster in Biology
Defense date2013
Abstract

Because of plants immobility, quick and effective regulations of transcription are of crucial importance for adaptation to environmental changes. Recently, it has been demonstrated for the first time that general epigenetic silencing of heterochromatin, through mechanisms that differ from those previously known, is transiently released after heat stress treatment. To determine these stress-specific epigenetic mechanisms, an EMS mutagenesis has been performed on mom1-6 lines containing a silencing reporter. M2 lines were then subjected to long heat stress and mutant candidates were selected according to their altered activation of marker transcription. Here I performed the screening and characterization of some of the most interesting mutants resulting from this first screening. I obtained results on DNA methylation status and expression of LUC, SDC, and SQN in stressed and unstressed plants and an initiated mapping of the mutations.

eng
Keywords
  • epigenetics
  • stress adaptation
  • arabidopsis thaliana
  • heterochromatic silencing
  • mutagenesis
  • release of silencing
Citation (ISO format)
ANTICO, Djan. Characterization of Mutants Involved in Release of Heterochromatic Silencing after a Long Heat Stress. 2013.
Main files (1)
Master thesis
accessLevelRestricted
Identifiers
  • PID : unige:28995
265views
6downloads

Technical informations

Creation07/27/2013 1:50:00 PM
First validation07/27/2013 1:50:00 PM
Update time03/14/2023 8:21:08 PM
Status update03/14/2023 8:21:08 PM
Last indexation01/29/2024 7:53:27 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack