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Title

Epithelial cells as active player in fibrosis: findings from an in vitro model

Authors
Ebeling, Martin
Weibel, Franziska
Araujo Del Rosario, Andrea
Hoflack, Jean Christophe
Pomposiello, Silvia
Published in PLOS ONE. 2013, vol. 8, no. 2, p. e56575
Abstract Kidney fibrosis, a scarring of the tubulo-interstitial space, is due to activation of interstitial myofibroblasts recruited locally or systemically with consecutive extracellular matrix deposition. Newly published clinical studies correlating acute kidney injury (AKI) to chronic kidney disease (CKD) challenge this pathological concept putting tubular epithelial cells into the spotlight. In this work we investigated the role of epithelial cells in fibrosis using a simple controlled in vitro system. An epithelial/mesenchymal 3D cell culture model composed of human proximal renal tubular cells and fibroblasts was challenged with toxic doses of Cisplatin, thus injuring epithelial cells. RT-PCR for classical fibrotic markers was performed on fibroblasts to assess their modulation toward an activated myofibroblast phenotype in presence or absence of that stimulus. Epithelial cell lesion triggered a phenotypical modulation of fibroblasts toward activated myofibroblasts as assessed by main fibrotic marker analysis. Uninjured 3D cell culture as well as fibroblasts alone treated with toxic stimulus in the absence of epithelial cells were used as control. Our results, with the caveats due to the limited, but highly controllable and reproducible in vitro approach, suggest that epithelial cells can control and regulate fibroblast phenotype. Therefore they emerge as relevant target cells for the development of new preventive anti-fibrotic therapeutic approaches.
Identifiers
PMID: 23457584
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Article (Published version) (339 Kb) - public document Free access
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Research groups Analyse protéomique et Analyse génomique des maladies rénales (659)
Chimie et protéomique clinique (270)
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MOLL, Solange et al. Epithelial cells as active player in fibrosis: findings from an in vitro model. In: PLOS ONE, 2013, vol. 8, n° 2, p. e56575. https://archive-ouverte.unige.ch/unige:28465

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Deposited on : 2013-06-10

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