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Scientific article
English

Highly sensitive capillary electrophoresis-mass spectrometry for rapid screening and accurate quantitation of drugs of abuse in urine

Contributors

Kohler, Isabelle; Schappler, Julie; Rudaz, Serge

Published in

Analytica chimica acta, vol. 780, p. 101-109

Publication date

2013

Abstract

The combination of capillary electrophoresis (CE) and mass spectrometry (MS) is particularly well adapted to bioanalysis due to its high separation efficiency, selectivity, and sensitivity; its short analytical time; and its low solvent and sample consumption. For clinical and forensic toxicology, a two-step analysis is usually performed: first, a screening step for compound identification, and second, confirmation and/or accurate quantitation in cases of presumed positive results. In this study, a fast and sensitive CE-MS workflow was developed for the screening and quantitation of drugs of abuse in urine samples. A CE with a time-of-flight MS (CE-TOF/MS) screening method was developed using a simple urine dilution and online sample preconcentration with pH-mediated stacking. The sample stacking allowed for a high loading capacity (20.5% of the capillary length), leading to limits of detection as low as 2 ng mL−1 for drugs of abuse. Compound quantitation of positive samples was performed by CE-MS/MS with a triple quadrupole MS equipped with an adapted triple-tube sprayer and an electrospray ionization (ESI) source. The CE-ESIMS/MS method was validated for two model compounds, cocaine (COC) and methadone (MTD), according to the Guidance of the Food and Drug Administration. The quantitative performance was evaluated for selectivity, response function, the lower limit of quantitation, trueness, precision, and accuracy. COC and MTD detection in urine samples was determined to be accurate over the range of 10–1000 ng mL−1 and 21–1000 ng mL−1, respectively.

Keywords

  • Bioanalysis
  • Capillary electrophoresis
  • PH-mediated stacking
  • Sheath liquid interface
  • Tandem mass spectrometry
  • Time-of-flight mass spectrometry

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

KOHLER, Isabelle, SCHAPPLER, Julie, RUDAZ, Serge. Highly sensitive capillary electrophoresis-mass spectrometry for rapid screening and accurate quantitation of drugs of abuse in urine. In: Analytica chimica acta, 2013, vol. 780, p. 101–109. doi: 10.1016/j.aca.2013.03.065

Main files (1)

Article (Published version)

Identifiers

Additional URL for this publication

http://linkinghub.elsevier.com/retrieve/pii/S000326701300473X

Journal ISSN

0003-2670
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Technical informations

Creation

30/05/2013 10:50:00

First validation

30/05/2013 10:50:00

Update

14/03/2023 20:17:53

Status update

14/03/2023 20:17:53

Last indexation

18/03/2025 09:21:22
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