Diagnostic utility of FLI-1 monoclonal antibody and dual-colour, break-apart probe fluorescence in situ (FISH) analysis in Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI-1 polyclonal antibodies

Mhawech-fauceglia, Paulette; Herrmann, François; Penetrante, R.; Beck, Amy; Sait, S.; Block, A. M.; Odunsi, K.; Fisher, J.; Balos, L.; Cheney, R. T.

doi:10.1111/j.1365-2559.2006.02535.x

Scientific article

English

Diagnostic utility of FLI-1 monoclonal antibody and dual-colour, break-apart probe fluorescence in situ (FISH) analysis in Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI-1 polyclonal antibodies

Contributors

Mhawech-fauceglia, Paulette; Herrmann, François; Penetrante, R.; Beck, Amy; Sait, S.; Block, A. M.; Odunsi, K.; Fisher, J.; Balos, L.; Cheney, R. T.

Published in

Histopathology, vol. 49, no. 6, p. 569-575

Publication date

2006

Abstract

Aims: To compare the sensitivity and specificity of the recently commercially available FLI-1 monoclonal (FLI-1m) antibody with the currently used antibodies [CD99 and FLI-1 polyclonal (FLI-1p)] in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET) and to determine the diagnostic value of the EWSR1 (22q12) dual-colour, break-apart rearrangement probe fluorescence in situ hybridization (FISH) technique. Materials and methods: Forty-three cases of well-documented EWS/PNET and 15 non-EWS/PNET cases were retrieved from the archival files. Immunohistochemistry (IHC) for FLI-1p, FLI-1m and FISH analysis was performed. Results: The most sensitive and specific test panel for the diagnosis of EWS/PNET is the combination of CD99 and FLI-1p. FISH had a very high specificity (100%) but only a moderate sensitivity (50%). Conclusion: The combination of CD99 and FLI-1p is the method of choice for the diagnosis of EWS/PNET. EWRS1 (22q12) dual-colour, break-apart rearrangement probe FISH should be used as a confirmatory test in addition to CD99 and FLI1-p due to its high specificity.

UNIGE affiliation entities

Faculté de médecine / Section de médecine clinique / Département de réadaptation et gériatrie

Citation (ISO format)

MHAWECH-FAUCEGLIA, Paulette et al. Diagnostic utility of FLI-1 monoclonal antibody and dual-colour, break-apart probe fluorescence in situ (FISH) analysis in Ewing’s sarcoma/primitive neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI-1 polyclonal antibodies. In: Histopathology, 2006, vol. 49, n° 6, p. 569–575. doi: 10.1111/j.1365-2559.2006.02535.x

Article

Identifiers

PID : unige:28155
DOI : 10.1111/j.1365-2559.2006.02535.x
PMID : 17163841

Journal ISSN

0309-0167

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28/05/2013 15:43:26

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Diagnostic utility of FLI-1 monoclonal antibody and dual-colour, break-apart probe fluorescence in situ (FISH) analysis in Ewing's sarcoma/primitive neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI-1 polyclonal antibodies

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Published in

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Abstract

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Citation (ISO format)

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First validation

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Status update

Last indexation