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Phosphorylation and stabilization of Arabidopsis MAP kinase phosphatase 1 in response to UV-B stress

Published in Journal of Biological Chemistry. 2013, vol. 288, no. 1, p. 480-486
Abstract MAP kinase phosphatases (MKPs) are important regulators of the activation levels and kinetics of MAP kinases. This is crucial for a large number of physiological processes during development and growth, as well as interactions with the environment, including the response to ultraviolet-B (UV-B) stress. Arabidopsis MKP1 is a key regulator of MAP kinases MPK3 and MPK6 in response to UV-B stress. However, virtually nothing is presently known about the post-translational regulation of plant MKPs in vivo. Here, we provide evidence that MKP1 is a phosphoprotein in vivo and that MKP1 accumulates in response to UV-B stress. Moreover, proteasome inhibitor experiments suggest that MKP1 is constantly turned-over under non-stress conditions and that MKP1 is stabilized upon stress treatment. Stress-responsive phosphorylation and stabilization of MKP1 demonstrate the post-translational regulation of a plant MKP in vivo, adding an additional regulatory layer to MAP kinase signaling in plants.
Keywords Amino Acid SequenceArabidopsis/enzymologyArabidopsis Proteins/genetics/metabolismDual Specificity Phosphatase 1/chemistry/metabolismGene Expression RegulationPlant/radiation effectsMAP Kinase Signaling SystemMitogen-Activated Protein Kinases/genetics/metabolismMolecular Sequence DataMutagenesisSite-DirectedPhenotypePhosphorylationPlant Proteins/metabolismPlantsGenetically ModifiedProtein ProcessingPost-TranslationalSignal TransductionUltraviolet Rays
PMID: 23188831
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Other version: http://www.jbc.org/content/288/1/480.abstract
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GONZALEZ BESTEIRO, Marina, ULM, Roman. Phosphorylation and stabilization of Arabidopsis MAP kinase phosphatase 1 in response to UV-B stress. In: Journal of Biological Chemistry, 2013, vol. 288, n° 1, p. 480-486. doi: 10.1074/jbc.M112.434654 https://archive-ouverte.unige.ch/unige:27986

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Deposited on : 2013-05-27

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