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Scientific article
English

Reversion of the Arabidopsis UV-B photoreceptor UVR8 to the homodimeric ground state

Publication date2013
Abstract

Plants require the UV-B photoreceptor UV resistance locus 8 (UVR8) for acclimation and survival in sunlight. Upon UV-B perception, UVR8 switches instantaneously from a homodimeric to monomeric configuration, which leads to interaction with the key signaling protein constitutively photomorphogenic 1 (COP1) and induction of UV-B-protective responses. Here, we show that UVR8 monomerization is reversible in vivo, restoring the homodimeric ground state. We also demonstrate that the UVR8-interacting proteins repressor of UV-B photomorphogenesis (RUP)1 and RUP2 mediate UVR8 redimerization independently of COP1. UVR8 redimerization consequently disrupts the UVR8-COP1 interaction, which halts signaling. Our results identify a key role of RUP1- and RUP2-mediated UVR8 redimerization in photoreceptor inactivation, a crucial process that regenerates reactivatable UVR8 homodimers.

Keywords
  • Arabidopsis/genetics/metabolism/radiation effects
  • Arabidopsis Proteins/chemistry/genetics/metabolism
  • Chromosomal Proteins
  • Non-Histone/chemistry/genetics/metabolism
  • Genes
  • Plant
  • Mutation
  • Photoreceptors
  • Plant/chemistry/genetics/metabolism
  • Protein Multimerization/radiation effects
  • Protein Structure
  • Quaternary/radiation effects
  • Repressor Proteins/genetics/metabolism
  • Signal Transduction/radiation effects
  • Ubiquitin-Protein Ligases/genetics/metabolism
  • Ultraviolet Rays
Citation (ISO format)
HEIJDE, Marc, ULM, Roman. Reversion of the Arabidopsis UV-B photoreceptor UVR8 to the homodimeric ground state. In: Proceedings of the National Academy of Sciences of the United States of America, 2013, vol. 110, n° 3, p. 1113–1118. doi: 10.1073/pnas.1214237110
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0027-8424
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