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In vitro evaluation of an RGD-functionalized chitosan derivative for enhanced cell adhesion

Falson, Françoise
Rousselle, Patricia
Published in Carbohydrate polymers. 2012, vol. 90, no. 4, p. 1494-500
Abstract Tissue repair is a spontaneous process that is initiated on wounding. However, if this complex mechanism is impaired or not sufficient the use of biomaterials might increase the chance of successful healing. In this view, an RGD-functionalized polymer was developed to promote dermal healing. A water-soluble chitosan derivative, carboxymethyl-trimethylchitosan (CM-TM-chitosan) was synthesized and GRGDS-moieties were grafted to the backbone at a concentration of 59 nmol/mg polymer to increase cell-biomaterial interaction. Tested in vitro with cultured human dermal fibroblasts, the developed polymer showed good biocompatibility and the initial adhesion was increased by 3-5 times due to the GRGDS-moieties. Moreover, cell spreading was specific to the interaction with GRGDS, giving a 12-fold increase of cells showing a fully spread morphology within 30 min. Overall, CM-TM-chitosan conjugated with GRGDS-peptides may prove useful as a biomaterial in wound healing.
Keywords Wound healingHuman dermal fibroblastChitosanRGD peptideCell adhesion
PMID: 22944407
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Research group Laboratoire de pharmacie galénique et de biopharmacie
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HANSSON, Annasara et al. In vitro evaluation of an RGD-functionalized chitosan derivative for enhanced cell adhesion. In: Carbohydrate polymers, 2012, vol. 90, n° 4, p. 1494-500. doi: 10.1016/j.carbpol.2012.07.020 https://archive-ouverte.unige.ch/unige:27316

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Deposited on : 2013-04-08

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