Scientific article

In vitro evaluation of an RGD-functionalized chitosan derivative for enhanced cell adhesion

Published inCarbohydrate polymers, vol. 90, no. 4, p. 1494-1500
Publication date2012

Tissue repair is a spontaneous process that is initiated on wounding. However, if this complex mechanism is impaired or not sufficient the use of biomaterials might increase the chance of successful healing. In this view, an RGD-functionalized polymer was developed to promote dermal healing. A water-soluble chitosan derivative, carboxymethyl-trimethylchitosan (CM-TM-chitosan) was synthesized and GRGDS-moieties were grafted to the backbone at a concentration of 59 nmol/mg polymer to increase cell-biomaterial interaction. Tested in vitro with cultured human dermal fibroblasts, the developed polymer showed good biocompatibility and the initial adhesion was increased by 3-5 times due to the GRGDS-moieties. Moreover, cell spreading was specific to the interaction with GRGDS, giving a 12-fold increase of cells showing a fully spread morphology within 30 min. Overall, CM-TM-chitosan conjugated with GRGDS-peptides may prove useful as a biomaterial in wound healing.

  • Wound healing
  • Human dermal fibroblast
  • Chitosan
  • RGD peptide
  • Cell adhesion
Citation (ISO format)
HANSSON, Annasara et al. In vitro evaluation of an RGD-functionalized chitosan derivative for enhanced cell adhesion. In: Carbohydrate polymers, 2012, vol. 90, n° 4, p. 1494–1500. doi: 10.1016/j.carbpol.2012.07.020
Main files (1)
Article (Published version)
ISSN of the journal0144-8617

Technical informations

Creation04/03/2013 9:36:00 AM
First validation04/03/2013 9:36:00 AM
Update time03/14/2023 8:07:48 PM
Status update03/14/2023 8:07:48 PM
Last indexation08/29/2023 6:09:57 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack