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Microelectrodes and their use to assess ion channel function

Edelman, Aleksander
Hug, Martin J
Published in Journal of Cystic Fibrosis. 2004, vol. 3 Suppl 2, p. 113-7
Abstract Impalement of living cells with microelectrodes (MEs) is a useful approach to measure a variety of biological parameters such as membrane potential (Vm), intracellular free ion concentrations and cell-to-cell communication. Despite such widespread applications, the number of trained researchers capable of using MEs is diminishing. In this article, we describe the fundamentals of the preparation and the proper use of MEs to study the physiology of transepithelial ion transport.
Keywords AnimalsBiological Transport/physiologyEpithelial Cells/physiologyFluorescent Dyes/diagnostic useIon Channel Gating/physiologyIon Channels/physiologyIon Transport/physiologyMicroelectrodes
PMID: 15463941
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Research group Mucoviscidose et jonctions GAP (229)
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EDELMAN, Aleksander, CHANSON, Marc, HUG, Martin J. Microelectrodes and their use to assess ion channel function. In: Journal of Cystic Fibrosis, 2004, vol. 3 Suppl 2, p. 113-7. doi: 10.1016/j.jcf.2004.05.024 https://archive-ouverte.unige.ch/unige:27041

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Deposited on : 2013-03-26

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