UNIGE document Scientific Article
previous document  unige:27030  next document
add to browser collection

Regulation of gap junctional communication in CFTR-expressing pancreatic epithelial cells

Published in Pflügers Archiv. 2001, vol. 443 Suppl 1, p. S81-4
Abstract Gap junction channels provide a pathway for coordinating multicellular activity. To evaluate the contribution of cell-to-cell communication in the function of epithelial cells, we studied the strength of gap junctional coupling in pancreatic acinar and duct cells exposed to agents known to elevate the intracellular concentration of Ca(2+) or cAMP. In acinar cells, we observed that maximal concentrations of acetylcholine evoked a biphasic increase in cytosolic Ca(2+) mobilization. The second sustained phase, which depends on Ca(2+) influx into the cell, was associated with the rapid closure of gap junction channels. In duct cells, stimulation of CFTR-dependent Cl(-) currents with cAMP analogs markedly increased gap junctional conductance in pairs of cells. Interestingly, cAMP had no effect on intercellular communication between cells harboring the DeltaF508 mutation of CFTR. An abnormal pattern of gap junctional coupling may contribute to the altered functions of tissues affected in cystic fibrosis.
Keywords Acetylcholine/pharmacologyAnimalsCalcium/metabolismCell Communication/drug effects/physiologyCyclic AMP/pharmacologyCystic Fibrosis/physiopathologyCystic Fibrosis Transmembrane Conductance Regulator/physiologyEpithelial Cells/physiologyGap Junctions/physiologyHumansMalePancreas/cytologyPancreatic Ducts/cytologyPatch-Clamp TechniquesRatsRats, Sprague-DawleyRats, Wistar
PMID: 11845309
Full text
Article (Published version) (116 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Research group Mucoviscidose et jonctions GAP (229)
(ISO format)
CHANSON, Marc, SUTER, Susanne. Regulation of gap junctional communication in CFTR-expressing pancreatic epithelial cells. In: Pflügers Archiv, 2001, vol. 443 Suppl 1, p. S81-4. doi: 10.1007/s004240100650 https://archive-ouverte.unige.ch/unige:27030

370 hits

0 download


Deposited on : 2013-03-26

Export document
Format :
Citation style :