UNIGE document Scientific Article
previous document  unige:27028  next document
add to browser collection
Title

Analytical strategies for the characterization of therapeutic monoclonal antibodies

Authors
Published in Trends in Analytical Chemistry. 2013, vol. 42, p. 74-83
Abstract Recombinant monoclonal antibodies (mAbs) have become particularly relevant for the treatment of autoimmune diseases or cancers. Because of their inherent complexity and for safety reasons, there is a need to develop powerful analytical methods to provide detailed characterizations of mAbs. The aim of the present review is to detail the state-of-the-art of analytical strategies for mAb characterization. It focuses on the most important separation techniques used in this field, specifically, the chromatographic and electrophoretic approaches and their combination with mass spectrometry (MS). Thanks to recent improvements in separation science and MS devices, mAbs can be analyzed more easily. However, there is still a need to find new approaches that avoid adsorption issues.
Keywords AdsorptionAnalytical strategyCapillary electrophoresis (CE)CharacterizationLiquid chromatography (LC)Mass spectrometryMonoclonal antibody (mAb)RecombinantSeparationTherapeutic monoclonal antibody
Identifiers
Full text
Structures
Citation
(ISO format)
FEKETE, Szabolcs et al. Analytical strategies for the characterization of therapeutic monoclonal antibodies. In: Trends in Analytical Chemistry, 2013, vol. 42, p. 74-83. doi: 10.1016/j.trac.2012.09.012 https://archive-ouverte.unige.ch/unige:27028

797 hits

6 downloads

Update

Deposited on : 2013-03-26

Export document
Format :
Citation style :