en
Scientific article
English

Nucleolin interacts with US11 protein of herpes simplex virus 1 and is involved in its trafficking

Published inJournal of virology, vol. 86, no. 3, p. 1449-1457
Publication date2012
Abstract

Herpes simplex virus type 1 (HSV-1) infection induces profound nucleolar modifications at the functional and organizational levels, including nucleolar invasion by several viral proteins. One of these proteins is US11, which exhibits several different functions and displays both cytoplasmic localization and clear nucleolar localization very similar to that of the major multifunctional nucleolar protein nucleolin. To determine whether US11 interacts with nucleolin, we purified US11 protein partners by coimmunoprecipitations using a tagged protein, Flag-US11. From extracts of cells expressing Flag-US11 protein, we copurified a protein of about 100 kDa that was further identified as nucleolin. In vitro studies have demonstrated that nucleolin interacts with US11 and that the C-terminal domain of US11, which is required for US11 nucleolar accumulation, is sufficient for interaction with nucleolin. This association was confirmed in HSV-1-infected cells. We found an increase in the nucleolar accumulation of US11 in nucleolin-depleted cells, thereby revealing that nucleolin could play a role in US11 nucleocytoplasmic trafficking through one-way directional transport out of the nucleolus. Since nucleolin is required for HSV-1 nuclear egress, the interaction of US11 with nucleolin may participate in the outcome of infection.

Keywords
  • Blotting, Far-Western
  • HeLa Cells
  • Humans
  • Immunoprecipitation
  • Phosphoproteins/metabolism
  • Protein Binding
  • Protein Transport
  • RNA, Small Interfering
  • RNA-Binding Proteins/metabolism
  • Viral Proteins/metabolism
Citation (ISO format)
GRECO, Anna et al. Nucleolin interacts with US11 protein of herpes simplex virus 1 and is involved in its trafficking. In: Journal of virology, 2012, vol. 86, n° 3, p. 1449–1457. doi: 10.1128/JVI.06194-11
Main files (1)
Article (Accepted version)
accessLevelRestricted
Identifiers
ISSN of the journal0022-538X
657views
0downloads

Technical informations

Creation03/14/2013 10:19:00 AM
First validation03/14/2013 10:19:00 AM
Update time03/14/2023 8:05:49 PM
Status update03/14/2023 8:05:48 PM
Last indexation05/02/2024 1:07:58 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack