Quantum key distribution is an application of quantum physics that allows two distant partners to securely distribute shared random secrets which they can use for secure message encryption, authentication or other communication tasks. In this thesis, I study and implement several concepts, protocols and their components, in particular single photon detectors. I evaluate its compatibility with fiber infrastructures with the aim to improve applicability, performance and profitability of quantum key distribution in different scenarios. I present superconducting single photon detectors implemented for long distance quantum communication, as well as semiconductor detectors employing rapid sine gating schemes. Wavelength-multiplexing of quantum and classical communication channels is demonstrated to stabilize a polarization-encoded quantum channel against birefringence fluctuations, and to realize quantum and encrypted communication over one fiber. An implementation of the coherent one-way protocol is presented which features a hardware distillation engine, wavelength-multiplexing, and an analysis of finite key effects and authentication costs.