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Expression of leptin receptor mRNA (long form splice variant) in the human cerebellum

Huguenin, C
Greggio, B
Published in Neuroreport. 1997, vol. 8, no. 14, p. 3123-6
Abstract Primers specific to the long isoform of leptin receptor (OB-Rb) mRNA were used in reverse transcriptase-linked polymerase chain reactions (RT-PCR) to investigate the expression of this receptor in the hypothalamus and cerebellum of human and rat. For both regions, we observed RT-PCR cDNAs as well as restriction enzyme cleavage fragments of expected sizes. Additionally, in situ hybridization of human cerebellum using two independent [35S]oligonucleotide probes complementary to the OB-Rb mRNA sequence revealed a prominent hybridization signal within the granular layer. Overall, our findings demonstrate the expression of OB-Rb mRNA in the cerebellum and suggest that in such a location, leptin receptors may mediate a function presumably not linked to body weight homeostasis.
Keywords AnimalsCarrier Proteins/geneticsCerebellum/metabolismHumansIn Situ HybridizationObesityRNA SplicingRNA, Messenger/biosynthesisRatsReceptors, Cell SurfaceReceptors, Cytokine/geneticsReceptors, Leptin
PMID: 9331926
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Research group Groupe Bouras Constantin (neuropsychiatrie) (1)
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SAVIOZ, Armand et al. Expression of leptin receptor mRNA (long form splice variant) in the human cerebellum. In: Neuroreport, 1997, vol. 8, n° 14, p. 3123-6. https://archive-ouverte.unige.ch/unige:26605

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Deposited on : 2013-03-06

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