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Substrate-Initiated Synthesis of Cell-Penetrating Poly(disulfide)s

Published inJournal of the American Chemical Society, vol. 135, no. 6, p. 2088-2091
Publication date2013
Abstract

Lessons from surface-initiated polymerization are applied to grow cell-penetrating poly(disulfide)s directly on substrates of free choice. Reductive depolymerization after cellular uptake should then release the native substrates and minimize toxicity. In the presence of thiolated substrates, propagators containing a strained disulfide from asparagusic or, preferably, lipoic acid and a guanidinium cation polymerize into poly(disulfide)s in less than 5 min at room temperature at pH 7. Substrate-initiated polymerization of cationic poly(disulfide)s and their depolymerization with dithiothreitol causes the appearance and disappearance of transport activity in fluorogenic vesicles. The same process is further characterized by gel-permeation chromatography and fluorescence resonance energy transfer.

Citation (ISO format)
BANG, Eun Kyoung et al. Substrate-Initiated Synthesis of Cell-Penetrating Poly(disulfide)s. In: Journal of the American Chemical Society, 2013, vol. 135, n° 6, p. 2088–2091. doi: 10.1021/ja311961k
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ISSN of the journal0002-7863
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Technical informations

Creation02/13/2013 9:16:00 AM
First validation02/13/2013 9:16:00 AM
Update time03/14/2023 8:03:11 PM
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