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Title

Rapid LC-MS/MS quantification of the major benzodiazepines and their metabolites on dried blood spots using a simple and cost-effective sample pretreatment

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Published in Bioanalysis. 2012, vol. 4, no. 11, p. 1337-50
Abstract Dried blood spots (DBS) sampling has gained popularity in the bioanalytical community as an alternative to conventional plasma sampling, as it provides numerous benefits in terms of sample collection and logistics. The aim of this work was to show that these advantages can be coupled with a simple and cost-effective sample pretreatment, with subsequent rapid LC-MS/MS analysis for quantitation of 15 benzodiazepines, six metabolites and three Z-drugs. For this purpose, a simplified offline procedure was developed that consisted of letting a 5-µl DBS infuse directly into 100 µl of MeOH, in a conventional LC vial.
Keywords Benzodiazepines/analysis/metabolism/standardsBlood Specimen Collection/economicsChromatographyHigh Pressure Liquid/standardsDried Blood Spot TestingHumansMethanol/chemistryTandem Mass Spectrometry/standardsTime Factors
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PMID: 22720652
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Research group Groupe Mangin Patrice (médecine légale) (862)
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DEGLON, Julien et al. Rapid LC-MS/MS quantification of the major benzodiazepines and their metabolites on dried blood spots using a simple and cost-effective sample pretreatment. In: Bioanalysis, 2012, vol. 4, n° 11, p. 1337-50. https://archive-ouverte.unige.ch/unige:26396

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Deposited on : 2013-02-18

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