en
Scientific article
English

Rapid LC-MS/MS quantification of the major benzodiazepines and their metabolites on dried blood spots using a simple and cost-effective sample pretreatment

Published inBioanalysis, vol. 4, no. 11, p. 1337-1350
Publication date2012
Abstract

Dried blood spots (DBS) sampling has gained popularity in the bioanalytical community as an alternative to conventional plasma sampling, as it provides numerous benefits in terms of sample collection and logistics. The aim of this work was to show that these advantages can be coupled with a simple and cost-effective sample pretreatment, with subsequent rapid LC-MS/MS analysis for quantitation of 15 benzodiazepines, six metabolites and three Z-drugs. For this purpose, a simplified offline procedure was developed that consisted of letting a 5-µl DBS infuse directly into 100 µl of MeOH, in a conventional LC vial.

Keywords
  • Benzodiazepines/analysis/metabolism/standards
  • Blood Specimen Collection/economics
  • Chromatography
  • High Pressure Liquid/standards
  • Dried Blood Spot Testing
  • Humans
  • Methanol/chemistry
  • Tandem Mass Spectrometry/standards
  • Time Factors
Citation (ISO format)
DEGLON, Julien et al. Rapid LC-MS/MS quantification of the major benzodiazepines and their metabolites on dried blood spots using a simple and cost-effective sample pretreatment. In: Bioanalysis, 2012, vol. 4, n° 11, p. 1337–1350. doi: 10.4155/bio.12.42
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1757-6199
618views
4downloads

Technical informations

Creation11/22/2012 1:43:00 PM
First validation11/22/2012 1:43:00 PM
Update time03/14/2023 8:02:53 PM
Status update03/14/2023 8:02:53 PM
Last indexation01/16/2024 1:12:25 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack