en
Doctoral thesis
English

Dissecting the molecular mechanism of antisense-RNA mediated gene silencing in yeast S. cerevisae

Defense date2012-07-09
Abstract

Long non-coding RNAs (LncRNAs) are important regulators of gene transcription. My thesis work aims at gaining more insights into the molecular mechanism of PHO84 antisense RNA-mediated silencing. For this purpose, I used the synthetic genetic array (SGA) procedure and found, in addition to previously identified histone modifying enzymes, histone chaperones and chromatin remodelling complexes to be potential players of this mechanism. In the second part of my thesis, I discuss the results we published showing that the silencing of PHO84 gene could be induced in a trans-model independently of the gene position. Furthermore we show that the Set1 methyltransferase is a key player of this mechanism. In conclusion, all our data highlight the importance of non-coding RNAs in mediating RNAi independent transcriptional gene silencing.

eng
Keywords
  • Antisense RNA
  • Non-coding RNA
  • Transcription
  • Silencing
  • Promoter
  • PHO84
  • Yeast
Citation (ISO format)
BEYROUTHY, Nissrine. Dissecting the molecular mechanism of antisense-RNA mediated gene silencing in yeast S. cerevisae. 2012. doi: 10.13097/archive-ouverte/unige:26393
Main files (1)
Thesis
accessLevelRestricted
Identifiers
549views
14downloads

Technical informations

Creation01/11/2013 4:30:00 PM
First validation01/11/2013 4:30:00 PM
Update time03/14/2023 8:02:51 PM
Status update03/14/2023 8:02:51 PM
Last indexation01/29/2024 7:39:25 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack