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RORβ induces barrel-like neuronal clusters in the developing neocortex

Shnider, Sara J
Tischfield, David J
Galazo, Maria J
Macklis, Jeffrey D
Published in Cerebral cortex. 2012, vol. 22, no. 5, p. 996-1006
Abstract Neurons in layer IV of the rodent whisker somatosensory cortex are tangentially organized in periodic clusters called barrels, each of which is innervated by thalamocortical axons transmitting sensory information from a single principal whisker, together forming a somatotopic map of the whisker pad. Proper thalamocortical innervation is critical for barrel formation during development, but the molecular mechanisms controlling layer IV neuron clustering are unknown. Here, we investigate the role in this mapping of the nuclear orphan receptor RORβ, which is expressed in neurons in layer IV during corticogenesis. We find that RORβ protein expression specifically increases in the whisker barrel cortex during barrel formation and that in vivo overexpression of RORβ is sufficient to induce periodic barrel-like clustering of cortical neurons. Remarkably, this clustering can be induced as early as E18, prior to innervation by thalamocortical afferents and whisker derived-input. At later developmental stages, these ectopic neuronal clusters are specifically innervated by thalamocortical axons, demonstrated by anterograde labeling from the thalamus and by expression of thalamocortical-specific synaptic markers. Together, these data indicate that RORβ expression levels control cytoarchitectural patterning of neocortical neurons during development, a critical process for the topographical mapping of whisker input onto the cortical surface.
Keywords AnimalsBody Patterning/physiologyFluorescent Antibody TechniqueMiceMiceTransgenicMicroscopyConfocalNeocortex/cytology/embryology/metabolismNeurogenesis/physiologyNeurons/cytology/metabolismNuclear Receptor Subfamily 1Group FMember 2/metabolismSomatosensory Cortex/cytology/embryology/metabolismVibrissae/innervation
PMID: 21799210
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Research group Neurobiologie développementale et plasticité (880)
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JABAUDON, Denis et al. RORβ induces barrel-like neuronal clusters in the developing neocortex. In: Cerebral cortex, 2012, vol. 22, n° 5, p. 996-1006. doi: 10.1093/cercor/bhr182 https://archive-ouverte.unige.ch/unige:26349

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Deposited on : 2013-02-11

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