UNIGE document Scientific Article
previous document  unige:26262  next document
add to browser collection
Title

Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves

Authors
Colom, Bartomeu
Poitelon, Yannick
Huang, Wenlong
Woodfin, Abigail
Averill, Sharon
Del Carro, Ubaldo
Zambroni, Desirée
Brain, Susan D
show hidden authors show all authors [1 - 15]
Published in FASEB Journal. 2012, vol. 26, no. 3, p. 1064-76
Abstract Junctional adhesion molecule-C (JAM-C) is an adhesion molecule expressed at junctions between adjacent endothelial and epithelial cells and implicated in multiple inflammatory and vascular responses. In addition, we recently reported on the expression of JAM-C in Schwann cells (SCs) and its importance for the integrity and function of peripheral nerves. To investigate the role of JAM-C in neuronal functions further, mice with a specific deletion of JAM-C in SCs (JAM-C SC KO) were generated. Compared to wild-type (WT) controls, JAM-C SC KO mice showed electrophysiological defects, muscular weakness, and hypersensitivity to mechanical stimuli. In addressing the underlying cause of these defects, nerves from JAM-C SC KO mice were found to have morphological defects in the paranodal region, exhibiting increased nodal length as compared to WTs. The study also reports on previously undetected expressions of JAM-C, namely on perineural cells, and in line with nociception defects of the JAM-C SC KO animals, on finely myelinated sensory nerve fibers. Collectively, the generation and characterization of JAM-C SC KO mice has provided unequivocal evidence for the involvement of SC JAM-C in the fine organization of peripheral nerves and in modulating multiple neuronal responses.
Keywords AnimalsBlottingWesternCalcitonin Gene-Related Peptide/metabolismCell Adhesion Molecules/deficiency/genetics/physiologyEndothelial Cells/metabolismEpithelial Cells/metabolismFemaleImmunoglobulins/deficiency/genetics/physiologyImmunohistochemistryMaleMiceMice129 StrainMiceInbred C57BLMiceKnockoutMiceTransgenicMicroscopyConfocalMicroscopyElectronMotor Neurons/metabolismMuscleSkeletal/cytology/injuries/metabolismNerve Fibers/metabolismPeripheral Nerves/cytology/metabolism/physiologyReflex/physiologySchwann Cells/metabolismSciatic Nerve/metabolism/physiology/ultrastructureSensory Receptor Cells/metabolism
Identifiers
PMID: 22090315
Full text
Article (Published version) (1.6 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Molécules d'adhésion et processus de migration cellulaire (167)
Citation
(ISO format)
COLOM, Bartomeu et al. Schwann cell-specific JAM-C-deficient mice reveal novel expression and functions for JAM-C in peripheral nerves. In: FASEB Journal, 2012, vol. 26, n° 3, p. 1064-76. https://archive-ouverte.unige.ch/unige:26262

202 hits

1 download

Update

Deposited on : 2013-02-06

Export document
Format :
Citation style :