Transient expression of cellular retinol-binding protein-1 during cardiac repair after myocardial infarction

Yu, Mengyue; Ishibashi-Ueda, Hatsue; Ohta-Ogo, Keiko; Gabbiani, Giulio; Yamagishi, Masakazu; Hayashi, Kenshi; Hirota, Seiichi; Bochaton-Piallat, Marie-Luce; Hao, Hiroyuki

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Title		Transient expression of cellular retinol-binding protein-1 during cardiac repair after myocardial infarction
Authors		Yu, Mengyue Ishibashi-Ueda, Hatsue Ohta-Ogo, Keiko Gabbiani, Giulio Yamagishi, Masakazu Hayashi, Kenshi Hirota, Seiichi Bochaton-Piallat, Marie-Luce Hao, Hiroyuki show all authors [1 - 9]
Published in		Pathology International. 2012, vol. 62, no. 4, p. 246-53
Abstract		Retinoic acid (RA) is a vitamin A derivative that exerts pleiotropic biological effects. Intracellular transport and metabolism of RA are regulated by cellular retinol-binding proteins (CRBP). CRBP-1 is transiently expressed in granulation tissue fibroblasts during wound healing; however, its role in cardiac remodeling remains unknown. A rat myocardial infarction (MI) model was established by ligation of the left coronary artery, and hearts were obtained at 3, 6, 15, 30 and 45 days after operation. Heart sections were examined immunohistochemically using anti-vimentin, anti-α-smooth muscle actin (α-SMA), anti-matrix metalloproteinase (MMP)-2, anti-MMP-9 and anti-CRBP-1 antibodies. Infarction involved 48.8 ± 3.6% of the left ventricle and was followed by an important cardiac remodeling. Vimentin-positive fibroblastic cells including α-SMA-positive myofibroblasts expressed CRBP-1 at 3-, 6-, and 15-days after MI. Expression of CRBP-1 reached a maximum at 6-days after infarction. Thereafter, CRBP-1 expression was dramatically decreased, showing a similar tendency to MMP expression. Human heart specimens of individuals with a recent myocardial infarction demonstrated presence of CRBP-1-positive fibroblasts by immunohistochemistry. We have demonstrated that CRBP-1 is transiently expressed by fibroblasts during cardiac remodeling. Our results suggest that CRBP-1 plays a role in ventricular remodeling after MI allegedly through its RA binding activity.
Keywords		Actins/metabolism — Aged — Animals — Biological Markers/metabolism — Disease Models — Animal — Fibroblasts/metabolism/pathology — Heart Ventricles/metabolism — Humans — Male — Matrix Metalloproteinase 2/metabolism — Matrix Metalloproteinase 9/metabolism — Myocardial Infarction/metabolism/pathology — Myocardium/metabolism/pathology — Myocytes — Cardiac/metabolism/pathology — Rats — Rats — Wistar — Retinol-Binding Proteins — Cellular/metabolism — Ventricular Remodeling/physiology — Vimentin/metabolism
Identifiers		DOI: 10.1111/j.1440-1827.2012.02802.x PMID: 22449228
Full text		Article (Published version) (1.4 MB) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Research group		Bochaton-Piallat Marie-Luce (pathologie et immunologie) (646)
Citation (ISO format)		YU, Mengyue et al. Transient expression of cellular retinol-binding protein-1 during cardiac repair after myocardial infarction. In: Pathology International, 2012, vol. 62, n° 4, p. 246-53. https://archive-ouverte.unige.ch/unige:26259