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Scientific article
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English

DEAD-box RNA helicases in gram-positive RNA decay

Published inMethods in enzymology, vol. 511, p. 369-383
Publication date2012
Abstract

DEAD-box RNA helicases are important players in eukaryotic and bacterial RNA metabolism. A helicase from Staphylococcus aureus was recently shown to affect RNA decay, most likely via its interaction with the proposed Gram-positive degradosome. Some, but not all, RNAs are stabilized when the helicase CshA is mutated, and among the affected RNAs is the agrBDCA mRNA, which is responsible for quorum sensing in S. aureus. We describe how the stabilization of agr mRNA (and others) can be measured and how to conduct assays to measure the effects of quorum-sensing defects, such as biofilm formation and hemolysin production.

Keywords
  • Endoribonucleases/metabolism
  • Multienzyme Complexes/metabolism
  • Polyribonucleotide Nucleotidyltransferase/metabolism
  • RNA Helicases/metabolism
  • RNA Stability/genetics/physiology
  • RNA
  • Bacterial/genetics/metabolism
  • RNA
  • Messenger/genetics/metabolism
  • Staphylococcus aureus/enzymology/metabolism
Citation (ISO format)
REDDER, Peter, LINDER, Patrick. DEAD-box RNA helicases in gram-positive RNA decay. In: Methods in enzymology, 2012, vol. 511, p. 369–383. doi: 10.1016/B978-0-12-396546-2.00017-6
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Article (Published version)
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ISSN of the journal0076-6879
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Creation06/11/2012 10:30:00
First validation06/11/2012 10:30:00
Update time14/03/2023 20:01:52
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