DEAD-box proteins are a large family of RNA helicases and are found in almost all organisms. They are implicated in all processes in which RNA is involved. In many bacteria, these proteins are induced for growth at low temperature and other abiotic stress conditions. The results presented here show that CshB, one of the two DEAD-box RNA helicases present in Staphyloccocus aureus, CshB is involved in the adaptation to cold and other abiotic stresses, such as oxidative stress and nutrients depletion. Moreover, it plays a more pleiotropic role as it is also implicated in several different biochemical pathways, including the switching to Small Colony Variant phenotype in an acid environment.