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Title

Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering
Authors
Boss, Daniel
Hoffmann, Annick
Rappaz, Benjamin
Depeursinge, Christian
Magistretti, Pierre
Van De Ville, Dimitri
Marquet, Pierre
Published in PLOS ONE. 2012, vol. 7, no. 8, p. e40667
Abstract Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of these remarkable biomechanical membrane properties altered in some pathological states including blood diseases. In particular the discussion over the thermal or metabolic origin of the CMF has led in the past to a large number of investigations and modeling. However, the origin of the CMF is still debated. In this article, we present an analysis of the CMF of RBCs by combining digital holographic microscopy (DHM) with an orthogonal subspace decomposition of the imaging data. These subspace components can be reliably identified and quantified as the eigenmode basis of CMF that minimizes the deformation energy of the RBC structure. By fitting the observed fluctuation modes with a theoretical dynamic model, we find that the CMF are mainly governed by the bending elasticity of the membrane and that shear and tension elasticities have only a marginal influence on the membrane fluctations of the discocyte RBC. Further, our experiments show that the role of ATP as a driving force of CMF is questionable. ATP, however, seems to be required to maintain the unique biomechanical properties of the RBC membrane that lead to thermally excited CMF.
Identifiers
PMID: 22899990
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Article (Published version) (441 Kb) - public document Free access
Structures
Faculté de médecine / Section de médecine clinique / Département de radiologie et informatique médicale
Research group Traitement d'images médicales (893)
Citation
(ISO format) 		BOSS, Daniel et al. Spatially-resolved eigenmode decomposition of red blood cells membrane fluctuations questions the role of ATP in flickering. In: PLOS ONE, 2012, vol. 7, n° 8, p. e40667. https://archive-ouverte.unige.ch/unige:26209

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Deposited on : 2013-02-05

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