A chloroplast repressible gene expression system in Chlamydomonas reinhardtii: a powerful tool to understand the function of essential plastid genes

Ramundo, Silvia

doi:10.13097/archive-ouverte/unige:25970

Doctoral thesis

English

A chloroplast repressible gene expression system in Chlamydomonas reinhardtii: a powerful tool to understand the function of essential plastid genes

Contributors

Ramundo, Silvia

Directors

Rochaix, Jean-David

Defense date

2012-04-18

Abstract

Chloroplast biogenesis requires coordination of gene expression between the organellar and nuclear genomes. Sequencing of chloroplast genomes from a large collection of green organisms has revealed the existence of approximately one hundred genes in this organelle present in multiple copies. In the green alga Chlamydomonas reinhardtii, many of these genes have been characterized thanks to its dispensable photo-autotrophic growth in acetate-containing medium and the successful development of plastid transformation methods based on its highly efficient chloroplast homologous recombination system. However, there are still a number of genes for which it has never been possible to disrupt all of their copies. These genes are believed to encode essential factors for growth and survival of the cell. The aim of this thesis was to establish an inducible/repressible expression system for chloroplast genes in Chlamydomonas reinhardtii and to explore its potential for elucidating the role of essential chloroplast genes.

Keywords

Chlamydomonas
Chloroplast
Essential genes
TPP-riboswitch
Gene regulation

UNIGE affiliation entities

Faculté des sciences / Section de biologie / Département de biologie moléculaire

Citation (ISO format)

RAMUNDO, Silvia. A chloroplast repressible gene expression system in Chlamydomonas reinhardtii: a powerful tool to understand the function of essential plastid genes. Doctoral Thesis, 2012. doi: 10.13097/archive-ouverte/unige:25970

Thesis

Identifiers

PID : unige:25970
DOI : 10.13097/archive-ouverte/unige:25970
URN : urn:nbn:ch:unige-259703
Thesis number : Sc. 4421

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23/01/2013 22:07:00

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23/01/2013 22:07:00

Update

14/03/2023 20:00:10

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14/03/2023 20:00:10

Last indexation

13/05/2025 16:09:02

A chloroplast repressible gene expression system in Chlamydomonas reinhardtii: a powerful tool to understand the function of essential plastid genes

Contributors

Directors

Defense date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Technical informations

Creation

First validation

Update

Status update

Last indexation