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Title

Artificial 64-residue HIV-1 enhancer-binding peptide is a potent inhibitor of viral replication in HIV-1-infected cells

Authors
Oufir, Mouhssin
Bisset, Leslie R
Hoffmann, Stefan R K
Xue, Gongda
Klauser, Stephan
Bergamaschi, Bianca
Böni, Jürg
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Published in Advances in Virology. 2011, vol. 2011, no. 165871, p. 1-13
Abstract An artificial HIV-1 enhancer-binding peptide was extended by nine consecutive arginine residues at the C-terminus and by the nuclear localization signal of SV40 large T antigen at the N-terminus. The resulting synthetic 64-residue peptide was found to bind to the two enhancers of the HIV-1 long terminal repeat, cross the plasma membrane and the nuclear envelope of human cells, and suppress the HIV-1 enhancer-controlled expression of a green fluorescent protein reporter gene. Moreover, HIV-1 replication is inhibited by this peptide in HIV-1-infected CEM-GFP cells as revealed by HIV-1 p24 ELISA and real-time RT-PCR of HIV-1 RNA. Rapid uptake of this intracellular stable and inhibitory peptide into the cells implies that this peptide may have the potential to attenuate HIV-1 replication in vivo.
Identifiers
PMID: 22312334
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Article (Published version) (2.6 MB) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Etiologie des pneumonies et marqueurs inflammatoires chez l'enfant fébrile (183)
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OUFIR, Mouhssin et al. Artificial 64-residue HIV-1 enhancer-binding peptide is a potent inhibitor of viral replication in HIV-1-infected cells. In: Advances in Virology, 2011, vol. 2011, n° 165871, p. 1-13. doi: 10.1155/2011/165871 https://archive-ouverte.unige.ch/unige:25776

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Deposited on : 2013-01-21

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