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Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage

CollaborationWith : Bosman, Alexis / Feki, Anis / Jaconi, Marisa
Published in Nature Biotechnology. 2011, vol. 29, no. 12, p. 1132-44
Abstract The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells.
Keywords Cell Differentiation/geneticsCell LineChromosomes, Human, Pair 20/geneticsClonal Evolution/geneticsDNA MethylationEmbryonic Stem Cells/cytologyEthnic Groups/geneticsGene Expression Regulation, DevelopmentalGenetic VariationGenotypeGrowth/geneticsHumansInduced Pluripotent Stem Cells/cytologyInhibitor of Differentiation Protein 1/genetics/metabolismPolymorphism, Single NucleotideRNA-Binding Proteins/genetics/metabolismSelection, Genetic/geneticsBcl-X Protein/genetics/metabolism
PMID: 22119741
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Research group Mécanismes de différentiation cellulaire des cellules cardiaques (536)
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INTERNATIONAL STEM CELL INITIATIVE. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. In: Nature Biotechnology, 2011, vol. 29, n° 12, p. 1132-44. doi: 10.1038/nbt.2051 https://archive-ouverte.unige.ch/unige:25638

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Deposited on : 2013-01-17

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