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Scientific article
Open access
English

Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology

Published inJournal of cell science, vol. 124, no. Pt 19, p. 3369-3380
Publication date2011
Abstract

An increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn(2+)-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 causes elongation and thinning of dendritic spines in the hippocampal neurons. These models are: a recently developed transgenic rat overexpressing autoactivating MMP-9, dissociated neuronal cultures, and organotypic neuronal cultures treated with recombinant autoactivating MMP-9. This dendritic effect is mediated by integrin β1 signalling. MMP-9 treatment also produces a change in the decay time of miniature synaptic currents; however, it does not change the abundance and localization of synaptic markers in dendritic protrusions. Our results, considered together with several recent studies, strongly imply that MMP-9 is functionally involved in synaptic remodelling.

Keywords
  • Animals
  • Antigens, CD29/metabolism
  • Cell Shape
  • Cells, Cultured
  • Chromatography, Affinity
  • Dendritic Spines/metabolism/physiology
  • Enzyme Assays
  • Excitatory Postsynaptic Potentials/drug effects
  • Hippocampus/cytology/metabolism
  • Matrix Metalloproteinase 9/isolation & purification/metabolism/pharmacology
  • Microscopy, Fluorescence
  • Nerve Tissue Proteins/metabolism
  • Patch-Clamp Techniques
  • Presynaptic Terminals/metabolism
  • Primary Cell Culture
  • Rats
  • Rats, Transgenic
  • Rats, Wistar
  • Recombinant Proteins/isolation & purification/metabolism/pharmacology
  • Tissue Culture Techniques
Citation (ISO format)
MICHALUK, Piotr et al. Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology. In: Journal of cell science, 2011, vol. 124, n° Pt 19, p. 3369–3380. doi: 10.1242/jcs.090852
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Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0021-9533
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392downloads

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