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Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology

Michaluk, Piotr
Wawrzyniak, Marcin
Alot, Przemyslaw
Szczot, Marcin
Wyrembek, Paulina
Mercik, Katarzyna
Medvedev, Nikolay
Wilczek, Ewa
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Published in Journal of cell science. 2011, vol. 124, no. Pt 19, p. 3369-80
Abstract An increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn(2+)-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 causes elongation and thinning of dendritic spines in the hippocampal neurons. These models are: a recently developed transgenic rat overexpressing autoactivating MMP-9, dissociated neuronal cultures, and organotypic neuronal cultures treated with recombinant autoactivating MMP-9. This dendritic effect is mediated by integrin β1 signalling. MMP-9 treatment also produces a change in the decay time of miniature synaptic currents; however, it does not change the abundance and localization of synaptic markers in dendritic protrusions. Our results, considered together with several recent studies, strongly imply that MMP-9 is functionally involved in synaptic remodelling.
Keywords AnimalsAntigens, CD29/metabolismCell ShapeCells, CulturedChromatography, AffinityDendritic Spines/metabolism/physiologyEnzyme AssaysExcitatory Postsynaptic Potentials/drug effectsHippocampus/cytology/metabolismMatrix Metalloproteinase 9/isolation & purification/metabolism/pharmacologyMicroscopy, FluorescenceNerve Tissue Proteins/metabolismPatch-Clamp TechniquesPresynaptic Terminals/metabolismPrimary Cell CultureRatsRats, TransgenicRats, WistarRecombinant Proteins/isolation & purification/metabolism/pharmacologyTissue Culture Techniques
PMID: 21896646
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Research group Plasticité des synapses excitatrices (76)
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MICHALUK, Piotr et al. Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology. In: Journal of cell science, 2011, vol. 124, n° Pt 19, p. 3369-80. doi: 10.1242/jcs.090852 https://archive-ouverte.unige.ch/unige:25597

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Deposited on : 2013-01-16

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