Self-Assembled Antibody Multimers through Peptide Nucleic Acid Conjugation

Kazane, Stephanie A.; Axup, Jun Y.; Kim, Chan Hyuk; Ciobanu, Mihai; Wold, Erik D.; Barluenga, Sofia; Hutchins, Benjamin A.; Schultz, Peter G.; Winssinger, Nicolas; Smider, Vaughn V.

doi:10.1021/ja309505c

Scientific article

English

Self-Assembled Antibody Multimers through Peptide Nucleic Acid Conjugation

Contributors

Kazane, Stephanie A.; Axup, Jun Y.; Kim, Chan Hyuk; Ciobanu, Mihai; Wold, Erik D.; Barluenga, Sofia; Hutchins, Benjamin A.; Schultz, Peter G.; Winssinger, Nicolas

; Smider, Vaughn V.

Published in

Journal of the American Chemical Society, vol. 135, no. 1, p. 340-346

Publication date

2013

Abstract

With the recent clinical success of bispecific antibodies, a strategy to rapidly synthesize and evaluate bispecific or higher order multispecific molecules could facilitate the discovery of new therapeutic agents. Here, we show that unnatural amino acids (UAAs) with orthogonal chemical reactivity can be used to generate site-specific antibody–oligonucleotide conjugates. These constructs can then be self-assembled into multimeric complexes with defined composition, valency, and geometry. With this approach, we generated potent bispecific antibodies that recruit cytotoxic T lymphocytes to Her2 and CD20 positive cancer cells, as well as multimeric antibody fragments with enhanced activity. This strategy should accelerate the synthesis and in vitro characterization of antibody constructs with unique specificities and molecular architectures.

UNIGE affiliation entities

Faculté des sciences / Section de chimie et biochimie / Département de chimie organique

Citation (ISO format)

KAZANE, Stephanie A. et al. Self-Assembled Antibody Multimers through Peptide Nucleic Acid Conjugation. In: Journal of the American Chemical Society, 2013, vol. 135, n° 1, p. 340–346. doi: 10.1021/ja309505c

Article (Published version)

Identifiers

PID : unige:25547
DOI : 10.1021/ja309505c

Journal ISSN

0002-7863

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09/01/2013 09:32:00

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09/01/2013 09:32:00

Update

13/10/2025 23:47:39

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14/03/2023 19:57:22

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01/07/2026 06:10:19

Self-Assembled Antibody Multimers through Peptide Nucleic Acid Conjugation

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

Citation (ISO format)

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Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation