UNIGE document Scientific Article
previous document  unige:25405  next document
add to browser collection

Alterations in lipid metabolism and thermogenesis with emergence of brown adipocytes in white adipose tissue in diet-induced obesity-resistant Lou/C rats

Published in American journal of physiology: endocrinology and metabolism. 2011, vol. 300, no. 6, p. E1146-57
Abstract Recent studies describe the Lou/C rat as a model of resistance to age- and diet-induced obesity and suggest a preferential channeling of nutrients toward utilization rather than storage under standard feeding conditions. The purpose of the present study was to evaluate lipid metabolism of Lou/C and Wistar rats under a high-fat (HF) diet. Four-month-old male Lou/C and Wistar animals were submitted to a 40% HF diet for 5-9 wk. Evolution of food intake, body weight, and body composition, hormonal parameters, and expression of key transcription factors and enzymes involved in lipid metabolism were determined. Wistar rats developed obesity after 5 wk of HF diet, as previously described. Among the various parameters measured, accumulation of intraperitoneal fat was particularly evident in HF-fed Wistar rats. In these animals, thermogenesis was, however, stimulated as a likely compensatory mechanism against the development of obesity. On the contrary, Lou/C animals failed to develop obesity under such a diet, and intraperitoneal fat, not including epididymal and retroperitoneal fat depots, was virtually absent. Enzyme measurements confirmed lipid utilization rather than storage, which was accompanied by the striking emergence of uncoupling protein-1, characteristic of brown adipocytes, in white adipose tissue, particularly in the subcutaneous depot.
Keywords Adipocytes, Brown/physiologyAdipose Tissue, White/cytology/physiologyAnimalsBlood Glucose/metabolismBlotting, WesternCalorimetry, IndirectDietDietary Fats/metabolism/pharmacologyFeeding Behavior/drug effectsGlucose Clamp TechniqueGlucose Tolerance TestIntestinal Absorption/genetics/physiologyLipid Metabolism/physiologyLiver/drug effects/metabolismMaleMuscle, Skeletal/drug effects/metabolismObesity/genetics/metabolismRNA/geneticsRatsRats, Inbred StrainsRats, WistarReverse Transcriptase Polymerase Chain ReactionThermogenesis/physiologyWeight Gain/drug effects
PMID: 21406614
Full text
Article (Published version) (1 MB) - document accessible for UNIGE members only Limited access to UNIGE
Research group Homéostasie métabolique (649)
(ISO format)
VEYRAT-DUREBEX, Christelle et al. Alterations in lipid metabolism and thermogenesis with emergence of brown adipocytes in white adipose tissue in diet-induced obesity-resistant Lou/C rats. In: American journal of physiology: endocrinology and metabolism, 2011, vol. 300, n° 6, p. E1146-57. doi: 10.1152/ajpendo.00575.2010 https://archive-ouverte.unige.ch/unige:25405

526 hits

0 download


Deposited on : 2013-01-11

Export document
Format :
Citation style :