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Title

Prenatal exposure to ethanol: a specific effect on the H19 gene in sperm

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Published in Reproductive Toxicology. 2011, vol. 31, no. 4, p. 507-12
Abstract Alcohol exposure during pregnancy induces a range of disorders in the offspring. Methylation changes in imprinted genes may play a role in the teratogenic effects of alcohol. We evaluated the possible effects of alcohol administration in pregnant mice on the methylation pattern of 5 imprinted genes (H19, Gtl2, Peg1, Snrpn and Peg3) in somatic and sperm cell DNAs of the male offspring. The effects observed were a 3% (p < 0.005) decrease in the number of methylated CpGs of H19 in the F1 offspring sperm, a 4% (p < 0.005) decrease in the number of methylated CpGs of H19 in the F2 offspring brain and a 26% (p < 0.05) decrease in the mean sperm concentration. CpGs 1 and 2 of the H19 CTCF-binding site 2 exhibited significant methylation percentage losses. H19 CTCF-binding sites are important for the regulation of Igf2 gene expression. The hypomethylation of H19 may contribute to the decreased spermatogenesis in the offspring.
Keywords Alcohol Drinking/adverse effectsAnimalsBrain/drug effects/embryology/metabolismCpG Islands/drug effectsDNA Methylation/drug effectsEthanol/toxicityFemaleGene Expression Regulation, Developmental/drug effectsGenomic Imprinting/drug effectsGestational AgeMaleMaternal ExposureMicePregnancyPrenatal Exposure Delayed EffectsRNA, Untranslated/geneticsSperm CountSpermatogenesis/drug effects/geneticsSpermatozoa/drug effects/metabolism
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PMID: 21382472
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Research groups Groupe Giacobino Ariane (génétique) (923)
Centre de Vaccinologie et d'Immunologie néonatale (177)
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STOUDER, Christelle, SOMM, Emmanuel, GIACOBINO, Ariane. Prenatal exposure to ethanol: a specific effect on the H19 gene in sperm. In: Reproductive Toxicology, 2011, vol. 31, n° 4, p. 507-12. https://archive-ouverte.unige.ch/unige:25388

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Deposited on : 2013-01-11

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