Prenatal exposure to ethanol: a specific effect on the H19 gene in sperm

Stouder, Christelle; Somm, Emmanuel; Giacobino, Ariane

doi:10.1016/j.reprotox.2011.02.009

Scientific article

English

Prenatal exposure to ethanol: a specific effect on the H19 gene in sperm

ContributorsStouder, Christelle; Somm, Emmanuel; Giacobino, Ariane

Published inReproductive toxicology, vol. 31, no. 4, p. 507-512

Publication date2011

Abstract

Alcohol exposure during pregnancy induces a range of disorders in the offspring. Methylation changes in imprinted genes may play a role in the teratogenic effects of alcohol. We evaluated the possible effects of alcohol administration in pregnant mice on the methylation pattern of 5 imprinted genes (H19, Gtl2, Peg1, Snrpn and Peg3) in somatic and sperm cell DNAs of the male offspring. The effects observed were a 3% (p < 0.005) decrease in the number of methylated CpGs of H19 in the F1 offspring sperm, a 4% (p < 0.005) decrease in the number of methylated CpGs of H19 in the F2 offspring brain and a 26% (p < 0.05) decrease in the mean sperm concentration. CpGs 1 and 2 of the H19 CTCF-binding site 2 exhibited significant methylation percentage losses. H19 CTCF-binding sites are important for the regulation of Igf2 gene expression. The hypomethylation of H19 may contribute to the decreased spermatogenesis in the offspring.

Keywords

Alcohol Drinking/adverse effects
Animals
Brain/drug effects/embryology/metabolism
CpG Islands/drug effects
DNA Methylation/drug effects
Ethanol/toxicity
Female
Gene Expression Regulation, Developmental/drug effects
Genomic Imprinting/drug effects
Gestational Age
Male
Maternal Exposure
Mice
Pregnancy
Prenatal Exposure Delayed Effects
RNA, Untranslated/genetics
Sperm Count
Spermatogenesis/drug effects/genetics
Spermatozoa/drug effects/metabolism

Affiliation

Research group

Citation (ISO format)

STOUDER, Christelle, SOMM, Emmanuel, GIACOBINO, Ariane. Prenatal exposure to ethanol: a specific effect on the H19 gene in sperm. In: Reproductive toxicology, 2011, vol. 31, n° 4, p. 507–512. doi: 10.1016/j.reprotox.2011.02.009

Article (Published version)

Identifiers

PID : unige:25388
DOI : 10.1016/j.reprotox.2011.02.009
PMID : 21382472

ISSN of the journal0890-6238

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Prenatal exposure to ethanol: a specific effect on the H19 gene in sperm

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