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Evaluation of the ability of Bifidobacterium longum to metabolize human intestinal mucus

Ruiz, Lorena
Gueimonde, Miguel
Salminen, Seppo
de los Reyes-Gavilán, Clara G
Margolles, Abelardo
Published in FEMS Microbiology Letters. 2011, vol. 314, no. 2, p. 125-30
Abstract The ability of Bifidobacterium longum to use intestinal mucus as a metabolizable source was characterized. Bifidobacterium longum biotype longum NCIMB8809 was grown in a chemically semi-defined medium supplemented with human intestinal mucus, and the cytoplasmic protein profiles and several glycosyl hydrolase activities were analysed and compared with those obtained from the same bacterium grown in the absence of mucus. We were able to identify 22 different proteins in the cytoplasmic fraction, of which nine displayed a different concentration in the presence of mucus. Among the proteins whose concentrations varied, we found specific enzymes that are involved in the response to different environmental conditions, and also proteins that mediate interaction with mucus in bacteria. Significant changes in some glycoside-hydrolysing activities were also detected. In addition, stable isotope labelling of amino acids in cell culture demonstrated that B. longum incorporates leucine from the glycoprotein matrix of mucin within its proteins. This study provides the first proteomic data regarding the interaction of B. longum with intestinal mucus, and contributes to the understanding of the behaviour of this intestinal species in its natural ecological niche.
Keywords Amino Acids/metabolismBacterial Proteins/analysisBifidobacterium/chemistry/growth & development/metabolismCulture Media/chemistryCytoplasm/chemistryGlycoproteins/metabolismGlycoside Hydrolases/metabolismHumansIsotopes/metabolismMucus/metabolismProteome/analysisStaining and Labeling/methods
PMID: 21105908
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Research group Groupe de Protéomique biomédicale (635)
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RUIZ, Lorena et al. Evaluation of the ability of Bifidobacterium longum to metabolize human intestinal mucus. In: FEMS Microbiology Letters, 2011, vol. 314, n° 2, p. 125-30. doi: 10.1111/j.1574-6968.2010.02159.x https://archive-ouverte.unige.ch/unige:25203

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Deposited on : 2013-01-09

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