UNIGE document Scientific Article
previous document  unige:25180  next document
add to browser collection
Title

Identification of human, rat and chicken ribosomal proteins by a combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry

Authors
Nguyen-Lefebvre, Anh Thu
Gonin-Giraud, Sandrine
Granger, Laure
Diaz, Jean-Jacques
Gandrillon, Olivier
show hidden authors show all authors [1 - 9]
Published in Journal of Proteomics. 2011, vol. 74, no. 2, p. 167-85
Abstract To identify the exact spot position of human, rat and chicken ribosomal proteins (RP) separated by two-dimensional polyacrylamide gel electrophoresis (2-DE), a 2-DE system was designed to separate RP with a pI>8.6 according to their charge in the first dimension and to their molecular mass in the second dimension. Individual proteins were excised from the gels and identified by mass spectrometry after digestion by trypsin. In addition, a mixture of purified RP from these three species was also analyzed by tandem mass tag spectrometry. By combining those two methods 74 RP from human, 76 from rat and 67 from chicken were identified according to the nomenclature initially defined for rat liver RP and by using the Swiss-Prot/trEMBL databases. Whereas human and rat RP were well described, most of RP from chicken were not characterized in databases, since 35 out of 67 chicken RP identified in this study were not listed yet. We propose here the first comprehensive description of chicken RP and their comparison to those from human and rat.
Keywords AnimalsChickensDatabases, FactualElectrophoresis, Gel, Two-Dimensional/methodsHeLa CellsHumansRatsRibosomal Proteins/analysis/chemistry/metabolismTandem Mass Spectrometry/methodsTrypsin/metabolismTumor Cells, Cultured
Identifiers
PMID: 21055487
Full text
Article (Published version) (755 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe de Protéomique biomédicale (635)
Citation
(ISO format)
NGUYEN-LEFEBVRE, Anh Thu et al. Identification of human, rat and chicken ribosomal proteins by a combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. In: Journal of Proteomics, 2011, vol. 74, n° 2, p. 167-85. https://archive-ouverte.unige.ch/unige:25180

173 hits

0 download

Update

Deposited on : 2013-01-09

Export document
Format :
Citation style :