Identification of human, rat and chicken ribosomal proteins by a combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry

Nguyen-Lefebvre, Anh Thu; Gonin-Giraud, Sandrine; Scherl, Alexander; Arboit, Patrizia; Granger, Laure; Sanchez, Jean-Charles; Diaz, Jean-Jacques; Gandrillon, Olivier; Madjar, Jean-Jacques

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Title		Identification of human, rat and chicken ribosomal proteins by a combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry
Authors		Nguyen-Lefebvre, Anh Thu Gonin-Giraud, Sandrine Scherl, Alexander Arboit, Patrizia Granger, Laure Sanchez, Jean-Charles Diaz, Jean-Jacques Gandrillon, Olivier Madjar, Jean-Jacques show all authors [1 - 9]
Published in		Journal of Proteomics. 2011, vol. 74, no. 2, p. 167-85
Abstract		To identify the exact spot position of human, rat and chicken ribosomal proteins (RP) separated by two-dimensional polyacrylamide gel electrophoresis (2-DE), a 2-DE system was designed to separate RP with a pI>8.6 according to their charge in the first dimension and to their molecular mass in the second dimension. Individual proteins were excised from the gels and identified by mass spectrometry after digestion by trypsin. In addition, a mixture of purified RP from these three species was also analyzed by tandem mass tag spectrometry. By combining those two methods 74 RP from human, 76 from rat and 67 from chicken were identified according to the nomenclature initially defined for rat liver RP and by using the Swiss-Prot/trEMBL databases. Whereas human and rat RP were well described, most of RP from chicken were not characterized in databases, since 35 out of 67 chicken RP identified in this study were not listed yet. We propose here the first comprehensive description of chicken RP and their comparison to those from human and rat.
Keywords		Animals — Chickens — Databases, Factual — Electrophoresis, Gel, Two-Dimensional/methods — HeLa Cells — Humans — Rats — Ribosomal Proteins/analysis/chemistry/metabolism — Tandem Mass Spectrometry/methods — Trypsin/metabolism — Tumor Cells, Cultured
Identifiers		DOI: 10.1016/j.jprot.2010.10.007 PMID: 21055487
Full text		Article (Published version) (755 Kb) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Research group		Groupe de Protéomique biomédicale (635)
Citation (ISO format)		NGUYEN-LEFEBVRE, Anh Thu et al. Identification of human, rat and chicken ribosomal proteins by a combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. In: Journal of Proteomics, 2011, vol. 74, n° 2, p. 167-85. https://archive-ouverte.unige.ch/unige:25180