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Scientific article
English

In silico modulation of apoptotic Bcl-2 proteins by mistletoe lectin-1: functional consequences of protein modifications

Contributors

Khwaja, Tasneem A.; Wajahat, Tayyaba; Ahmad, Ishtiaq; Hoessli, Daniel; Walker-Nasir, Evelyne; Kaleem, Afshan; Qazi, Wajahat M.; Shakoori, Abdul R.; Nasir-Ud Din, Nasir-Ud

Published in

Journal of cellular biochemistry, vol. 103, no. 2, p. 479-491

Publication date

2008

Abstract

The mistletoe lectin-1 (ML-1) modulates tumor cell apoptosis by triggering signaling cascades through the complex interplay of phosphorylation and O-linked N-acetylglucosamine (O-GlcNAc) modification in pro- and anti-apoptotic proteins. In particular, ML-1 is predicted to induce dephosphorylation of Bcl-2-family proteins and their alternative O-GlcNAc modification at specific, conserved Ser/Thr residues. The sites for phosphorylation and glycosylation were predicted and analyzed using Netphos 2.0 and YinOYang 1.2. The involvement of modified Ser/Thr, and among them the potential Yin Yang sites that may undergo both types of posttranslational modification, is proposed to mediate apoptosis modulation by ML-1.

Keywords

  • Amino Acid Sequence
  • Animals
  • Apoptosis/drug effects
  • Cats
  • Cattle
  • Conserved Sequence
  • Cricetinae
  • Cricetulus
  • Deer
  • Dogs
  • Glycosylation/drug effects
  • Humans
  • Mice
  • Molecular Sequence Data
  • Phosphorylation/drug effects
  • Plant Preparations/pharmacology
  • Plant Proteins/pharmacology
  • Protein Processing, Post-Translational/drug effects
  • Proto-Oncogene Proteins c-bcl-2/chemistry/drug effects/physiology
  • Ribosome Inactivating Proteins, Type 2/pharmacology
  • Sequence Alignment
  • Sheep
  • Species Specificity
  • Toxins, Biological/pharmacology
  • Bcl-2-Associated X Protein/chemistry/drug effects/physiology
  • Bcl-Associated Death Protein/chemistry/drug effects/physiology

UNIGE affiliation entities

Citation (ISO format)

KHWAJA, Tasneem A. et al. In silico modulation of apoptotic Bcl-2 proteins by mistletoe lectin-1: functional consequences of protein modifications. In: Journal of cellular biochemistry, 2008, vol. 103, n° 2, p. 479–491. doi: 10.1002/jcb.21412

Main files (1)

Article (Accepted version)

Identifiers

Journal ISSN

0730-2312
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Technical informations

Creation

25/08/2009 17:05:00

First validation

25/08/2009 17:05:00

Update

26/03/2026 10:32:36

Status update

26/03/2026 10:32:36

Last indexation

26/03/2026 10:37:44
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