UNIGE document Scientific Article
previous document  unige:24793  next document
add to browser collection
Title

The junctional adhesion molecule JAM-C regulates polarized transendothelial migration of neutrophils in vivo

Authors
Woodfin, Abigail
Voisin, Mathieu-Benoit
Beyrau, Martina
Colom, Bartomeu
Caille, Dorothée
Diapouli, Frantzeska-Maria
Nash, Gerard B
Chavakis, Triantafyllos
show hidden authors show all authors [1 - 13]
Published in Nature Immunology. 2011, vol. 12, no. 8, p. 761-9
Abstract The migration of neutrophils into inflamed tissues is a fundamental component of innate immunity. A decisive step in this process is the polarized migration of blood neutrophils through endothelial cells (ECs) lining the venular lumen (transendothelial migration (TEM)) in a luminal-to-abluminal direction. By real-time confocal imaging, we found that neutrophils had disrupted polarized TEM ('hesitant' and 'reverse') in vivo. We noted these events in inflammation after ischemia-reperfusion injury, characterized by lower expression of junctional adhesion molecule C (JAM-C) at EC junctions, and they were enhanced by blockade or genetic deletion of JAM-C in ECs. Our results identify JAM-C as a key regulator of polarized neutrophil TEM in vivo and suggest that reverse TEM of neutrophils can contribute to the dissemination of systemic inflammation.
Keywords AnimalsCell Adhesion Molecules/immunologyEndothelium, Vascular/cytology/immunology/pathologyImage Processing, Computer-AssistedImmunoglobulins/immunologyInflammation/immunology/pathologyMiceMicroscopy, ConfocalNeutrophils/immunologyReperfusion Injury/immunology/pathologyTransendothelial and Transepithelial Migration/immunology
Identifiers
PMID: 21706006
Full text
Article (Published version) (1.7 MB) - public document Free access
Structures
Research groups Couplage cellulaire et connexines (136)
Molécules d'adhésion et processus de migration cellulaire (167)
Citation
(ISO format)
WOODFIN, Abigail et al. The junctional adhesion molecule JAM-C regulates polarized transendothelial migration of neutrophils in vivo. In: Nature Immunology, 2011, vol. 12, n° 8, p. 761-9. https://archive-ouverte.unige.ch/unige:24793

249 hits

101 downloads

Update

Deposited on : 2012-12-20

Export document
Format :
Citation style :