The junctional adhesion molecule JAM-C regulates polarized transendothelial migration of neutrophils in vivo

Woodfin, Abigail; Voisin, Mathieu-Benoit; Beyrau, Martina; Colom, Bartomeu; Caille, Dorothée; Diapouli, Frantzeska-Maria; Nash, Gerard B; Chavakis, Triantafyllos; Albelda, Steven M; Rainger, G Ed; Meda, Paolo; Imhof, Beat; Nourshargh, Sussan

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Title		The junctional adhesion molecule JAM-C regulates polarized transendothelial migration of neutrophils in vivo
Authors		Woodfin, Abigail Voisin, Mathieu-Benoit Beyrau, Martina Colom, Bartomeu Caille, Dorothée Diapouli, Frantzeska-Maria Nash, Gerard B Chavakis, Triantafyllos Albelda, Steven M Rainger, G Ed Meda, Paolo Imhof, Beat Nourshargh, Sussan show all authors [1 - 13]
Published in		Nature Immunology. 2011, vol. 12, no. 8, p. 761-9
Abstract		The migration of neutrophils into inflamed tissues is a fundamental component of innate immunity. A decisive step in this process is the polarized migration of blood neutrophils through endothelial cells (ECs) lining the venular lumen (transendothelial migration (TEM)) in a luminal-to-abluminal direction. By real-time confocal imaging, we found that neutrophils had disrupted polarized TEM ('hesitant' and 'reverse') in vivo. We noted these events in inflammation after ischemia-reperfusion injury, characterized by lower expression of junctional adhesion molecule C (JAM-C) at EC junctions, and they were enhanced by blockade or genetic deletion of JAM-C in ECs. Our results identify JAM-C as a key regulator of polarized neutrophil TEM in vivo and suggest that reverse TEM of neutrophils can contribute to the dissemination of systemic inflammation.
Keywords		Animals — Cell Adhesion Molecules/immunology — Endothelium, Vascular/cytology/immunology/pathology — Image Processing, Computer-Assisted — Immunoglobulins/immunology — Inflammation/immunology/pathology — Mice — Microscopy, Confocal — Neutrophils/immunology — Reperfusion Injury/immunology/pathology — Transendothelial and Transepithelial Migration/immunology
Identifiers		DOI: 10.1038/ni.2062 PMID: 21706006
Full text		Article (Published version) (1.7 MB) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme
Research groups		Couplage cellulaire et connexines (136) Molécules d'adhésion et processus de migration cellulaire (167)
Citation (ISO format)		WOODFIN, Abigail et al. The junctional adhesion molecule JAM-C regulates polarized transendothelial migration of neutrophils in vivo. In: Nature Immunology, 2011, vol. 12, n° 8, p. 761-9. https://archive-ouverte.unige.ch/unige:24793