Scientific article

Brain extracellular fluid protein changes in acute stroke patients

Published inJournal of proteome research, vol. 10, no. 3, p. 1043-1051
Publication date2011

In vivo human brain extracellular fluids (ECF) of acute stroke patients were investigated to assess the changes in protein levels associated with ischemic damages. Microdialysates (MDs) from the infarct core (IC), the penumbra (P), and the unaffected contralateral (CT) brain regions of patients suffering an ischemic stroke (n = 6) were compared using a shotgun proteomic approach based on isobaric tagging and mass spectrometry. Quantitative analysis showed 53 proteins with increased amounts in the IC or P with respect to the CT samples. Glutathione S-transferase P (GSTP1), peroxiredoxin-1 (PRDX1), and protein S100-B (S100B) were further assessed with ELISA on the blood of unrelated control (n = 14) and stroke (n = 14) patients. Significant increases of 8- (p = 0.0002), 20- (p = 0.0001), and 11-fold (p = 0.0093) were found, respectively. This study highlights the value of ECF as an efficient source to further discover blood stroke markers.

  • Adult
  • Animals
  • Biological Markers/analysis
  • Brain/pathology
  • Brain Chemistry
  • Extracellular Fluid/chemistry
  • Female
  • Humans
  • Immunoassay/methods
  • Male
  • Microdialysis
  • Middle Aged
  • Proteins/analysis
  • Stroke/metabolism/pathology
Citation (ISO format)
DAYON, Loic et al. Brain extracellular fluid protein changes in acute stroke patients. In: Journal of proteome research, 2011, vol. 10, n° 3, p. 1043–1051. doi: 10.1021/pr101123t
Main files (1)
Article (Published version)
ISSN of the journal1535-3893

Technical informations

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