NLRX1/NOD5 belongs to the NOD clade of the NOD-like receptor (NLR) family. NLRs have emerged as key intracellular sensors for pathogen-derived molecules and endogenous danger signals to activate innate immune responses and inflammation. However, the function of most of these receptors still remains unclear. Our data indicate that NOD5 deficiency does not affect RLR signalling in vitro and in vivo, at least under the conditions tested.