en
Scientific article
English

Exposure to CuO Nanoparticles Changes the Fatty Acid Composition of Protozoa Tetrahymena thermophila

Published inEnvironmental science & technology, vol. 45, no. 15, p. 6617-6624
Publication date2011
Abstract

In the current study, the toxicity mechanismof nanosized CuO (nCuO) to the freshwater ciliated protozoa Tetrahymena thermophila was studied. Changes in fatty acid profile, lipid peroxidation metabolites and reactive oxygen species (ROS) were measured. Bulk CuO and CuSO4 served as controls for size and solubility and 3,5-dichorophenol (3,5-DCP) as a control for a chemical known to directly affect the membrane composition. Exposure to all copper compounds induced the generation of ROS, whereas nCuO was most potent. The latter effect was not solely explained by solubilized Cu-ions and was apparently particle-related. 24 h exposure of protozoa to 80 mg/L of nCuO (EC50) significantly decreased the proportion of two major unsaturated fatty acids (UFA) (C18:3 cis-6,9,12, C18:2 cis-9,12), while it increased the relative amount of two saturated fatty acids (SFA) (C18:0, C16:0). Analogous effect was not observed when protozoa were exposed to equitoxic suspensions of bulk CuO, Cu-ions or 3,5-DCP. As changes in the UFA:SFA upon exposure of protozoa to nCuO were not detected at 2 h exposure and no simultaneous dose- or time-dependent lipid peroxidation occurred, it is likely that one of the adaptation mechanisms of protozoa to nCuO was lowering membrane fluidity by the inhibition of de novo synthesis of fatty acid desaturases. This is the first study of the effects of nanoparticles on the membrane fatty acid composition.

Affiliation Not a UNIGE publication
Citation (ISO format)
MORTIMER, Monika et al. Exposure to CuO Nanoparticles Changes the Fatty Acid Composition of Protozoa Tetrahymena thermophila. In: Environmental science & technology, 2011, vol. 45, n° 15, p. 6617–6624. doi: 10.1021/es201524q
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0013-936X
529views
0downloads

Technical informations

Creation11/12/2012 11:11:00 AM
First validation11/12/2012 11:11:00 AM
Update time03/14/2023 5:45:00 PM
Status update03/14/2023 5:45:00 PM
Last indexation01/16/2024 12:30:52 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack